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Involvement of Pre-limbic Cortex-Nucleus accumbens projections in Context-Induced alcohol seeking.
Bianchi, Paula Cristina; Palombo, Paola; Engi, Sheila Antonagi; Carneiro de Oliveira, Paulo Eduardo; Tavares, Gabrielle Emily Boaventura; Anjos-Santos, Alexia; Yokoyama, Thais Suemi; Planeta, Cleopatra da Silva; Cruz, Fábio Cardoso; Leão, Rodrigo Molini.
Afiliação
  • Bianchi PC; Laboratory of Behavioral Neuroscience, Paulista Medicine School, Universidade Federal de São Paulo-UNIFESP, São Paulo, SP, Brazil.
  • Palombo P; Laboratory of Behavioral Neuroscience, Paulista Medicine School, Universidade Federal de São Paulo-UNIFESP, São Paulo, SP, Brazil.
  • Engi SA; Laboratory of Behavioral Neuroscience, Paulista Medicine School, Universidade Federal de São Paulo-UNIFESP, São Paulo, SP, Brazil.
  • Carneiro de Oliveira PE; Laboratory of Neuropsychopharmacology, School of Pharmaceutical Sciences, São Paulo State University, UNESP, Araraquara, SP, Brazil.
  • Tavares GEB; Laboratory of Behavioral Neuroscience, Paulista Medicine School, Universidade Federal de São Paulo-UNIFESP, São Paulo, SP, Brazil.
  • Anjos-Santos A; Laboratory of Behavioral Neuroscience, Paulista Medicine School, Universidade Federal de São Paulo-UNIFESP, São Paulo, SP, Brazil.
  • Yokoyama TS; Laboratory of Behavioral Neuroscience, Paulista Medicine School, Universidade Federal de São Paulo-UNIFESP, São Paulo, SP, Brazil.
  • Planeta CDS; Laboratory of Neuropsychopharmacology, School of Pharmaceutical Sciences, São Paulo State University, UNESP, Araraquara, SP, Brazil.
  • Cruz FC; Laboratory of Behavioral Neuroscience, Paulista Medicine School, Universidade Federal de São Paulo-UNIFESP, São Paulo, SP, Brazil.
  • Leão RM; Laboratory of Pharmacology, Biomedical Sciences Institute, Department of Pharmacology, Federal University of Uberlândia, Uberlândia, MG, Brazil; Graduate Program in Genetics and Biochemistry, Institute of Biotechnology, Federal University of Uberlândia/MG, Brazil. Electronic address: rodrigoleao@ufu
Brain Res ; 1841: 149086, 2024 Oct 15.
Article em En | MEDLINE | ID: mdl-38876319
ABSTRACT
Alcohol use disorder (AUD) remains a critical public health issue worldwide, characterized by high relapse rates often triggered by contextual cues. This research investigates the neural mechanisms behind context-induced reinstatement of alcohol-seeking behavior, focusing on the nucleus accumbens and its interactions with the prelimbic cortex, employing Male Long-Evans rats in an ABA renewal model. In our experimental setup, rats were trained to self-administer 10 % ethanol in Context A, followed by extinction of lever pressing in the presence of discrete cues in Context B. The context-induced reinstatement of ethanol-seeking was then assessed by re-exposing rats to Context A or B under extinction conditions, aiming to simulate the environmental cues' influence on relapse behaviors. Three experiments were conducted Experiment 1 utilized Fos-immunohistochemistry to examine neuronal activation in the nucleus accumbens; Experiment 2 applied the baclofen + muscimol inactivation technique to probe the functional importance of the nucleus accumbens core; Experiment 3 used Fos-immunofluorescence along with Retrobeads injection to investigate activation of neurons projecting from the prelimbic cortex to the nucleus accumbens core. Our findings revealed significant increases in Fos-immunoreactive nuclei within the nucleus accumbens core and shell during the reinstatement phase in Context A, underscoring the environment's potent effect on ethanol-seeking behavior. Additionally, inactivation of the nucleus accumbens core markedly reduced reinstatement, and there was a notable activation of neurons from the prelimbic cortex to the nucleus accumbens core in the ethanol-associated context. These results highlight the critical role of the nucleus accumbens core and its corticostriatal projections in the neural circuitry underlying context-driven ethanol seeking.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ratos Long-Evans / Etanol / Extinção Psicológica / Comportamento de Procura de Droga / Núcleo Accumbens Limite: Animals Idioma: En Revista: Brain Res Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ratos Long-Evans / Etanol / Extinção Psicológica / Comportamento de Procura de Droga / Núcleo Accumbens Limite: Animals Idioma: En Revista: Brain Res Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda