Platelet-rich fibrin (PRF) modified nano-hydroxyapatite/chitosan/gelatin/alginate scaffolds increase adhesion and viability of human dental pulp stem cells (DPSC) and osteoblasts derived from DPSC.

Anaya-Sampayo, Lina María; García-Robayo, Dabeiba Adriana; Roa, Nelly S; Rodriguez-Lorenzo, Luis Maria; Martínez-Cardozo, Constanza

Anaya-Sampayo, Lina María; García-Robayo, Dabeiba Adriana; Roa, Nelly S; Rodriguez-Lorenzo, Luis Maria; Martínez-Cardozo, Constanza.

Afiliação

Anaya-Sampayo LM; Dental Research Center, School of Dentistry, Pontificia Universidad Javeriana, Bogotá, Colombia.
García-Robayo DA; Dental Research Center, School of Dentistry, Pontificia Universidad Javeriana, Bogotá, Colombia. Electronic address: garciad@javeriana.edu.co.
Roa NS; Dental Research Center, School of Dentistry, Pontificia Universidad Javeriana, Bogotá, Colombia.
Rodriguez-Lorenzo LM; Department of Polymeric Nanomaterials and Biomaterials, Institute Science and Technology of Polymers (ICTP-CSIC), Madrid, Spain.
Martínez-Cardozo C; Faculty of Dentistry, Universidad de los Andes, Chile. Electronic address: cemartinezc@uandes.cl.

Int J Biol Macromol ; 273(Pt 1): 133064, 2024 Jul.

Article em En | MEDLINE | ID: mdl-38866288

ABSTRACT

ABSTRACT

Bone tissue regeneration strategies have incorporated the use of natural polymers, such as hydroxyapatite (nHA), chitosan (CH), gelatin (GEL), or alginate (ALG). Additionally, platelet concentrates, such as platelet-rich fibrin (PRF) have been suggested to improve scaffold biocompatibility. This study aimed to develop scaffolds composed of nHA, GEL, and CH, with or without ALG and lyophilized PRF, to evaluate the scaffold's properties, growth factor release, and dental pulp stem cells (DPSC), and osteoblast (OB) derived from DPSC viability. Four scaffold variations were synthesized and lyophilized. Then, degradation, swelling profiles, and morphological analysis were performed. Furthermore, PDGF-BB and FGF-B growth factors release were quantified by ELISA, and cytotoxicity and cell viability were evaluated. The swelling and degradation profiles were similar in all scaffolds, with pore sizes ranging between 100 and 250 µm. FGF-B and PDGF-BB release was evidenced after 24 h of scaffold immersion in cell culture medium. DPSC and OB-DPSC viability was notably increased in PRF-supplemented scaffolds. The nHA-CH-GEL-PRF scaffold demonstrated optimal physical-biological characteristics for stimulating DPSC and OB-DPSC cell viability. These results suggest lyophilized PRF improves scaffold biocompatibility for bone tissue regeneration purposes.

Assuntos

Alginatos; Sobrevivência Celular; Quitosana; Polpa Dentária; Durapatita; Gelatina; Osteoblastos; Fibrina Rica em Plaquetas; Células-Tronco; Alicerces Teciduais; Humanos; Polpa Dentária/citologia; Quitosana/química; Quitosana/farmacologia; Gelatina/química; Fibrina Rica em Plaquetas/química; Fibrina Rica em Plaquetas/metabolismo; Alicerces Teciduais/química; Células-Tronco/efeitos dos fármacos; Células-Tronco/citologia; Células-Tronco/metabolismo; Sobrevivência Celular/efeitos dos fármacos; Durapatita/química; Durapatita/farmacologia; Alginatos/química; Alginatos/farmacologia; Osteoblastos/efeitos dos fármacos; Osteoblastos/citologia; Adesão Celular/efeitos dos fármacos; Engenharia Tecidual/métodos; Células Cultivadas

Palavras-chave

3D scaffolds; Adult stem cells; Bone regeneration; Cell survival; Platelet-rich fibrin

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Osteoblastos / Células-Tronco / Sobrevivência Celular / Durapatita / Polpa Dentária / Quitosana / Alginatos / Alicerces Teciduais / Fibrina Rica em Plaquetas / Gelatina Limite: Humans Idioma: En Revista: Int J Biol Macromol Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Colômbia País de publicação: Holanda

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