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Whole-genome sequencing analysis of multidrug-resistant Serratia marcescens isolates in an intensive care unit in Brazil.
Cruz, Thalita Pps; Azevedo, Francisco Ksf; Pepato, Marco A; Rizzo, Paula S; Souto, Francisco Jd; Brenig, Bertram; Góes-Neto, Aristóteles; Soares, Siomar C; Ramos, Rommel Tj; Azevedo, Vasco Ac; Costa, Mateus M; Broetto, Leonardo; Nakazato, Luciano; Dutra, Valéria.
Afiliação
  • Cruz TP; Laboratory of Veterinary Microbiology and Molecular Biology, Veterinary Hospital of Federal University of Mato Grosso, Cuiabá, MT, Brazil.
  • Azevedo FK; Department of Internal Medicine, Federal University of Mato Grosso, Cuiabá, MT, Brazil.
  • Pepato MA; Department of Internal Medicine, Federal University of Mato Grosso, Cuiabá, MT, Brazil.
  • Rizzo PS; Department of Internal Medicine, Federal University of Mato Grosso, Cuiabá, MT, Brazil.
  • Souto FJ; Department of Internal Medicine, Federal University of Mato Grosso, Cuiabá, MT, Brazil.
  • Brenig B; Institute of Veterinary Medicine, University of Göttingen, Göttingen, Niedersachsen, Germany.
  • Góes-Neto A; Laboratory of Molecular Computational Biology of Fungi (LBMCF), Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
  • Soares SC; Department of Microbiology, Immunology, and Parasitology, Federal University of Triangle Miner, Uberaba, Brazil.
  • Ramos RT; Center of Genomics and Systems Biology, Institute of Biological Sciences, Federal University of Pará (UFPA), Belém, Pará, Brazil.
  • Azevedo VA; Laboratory of Cellular and Molecular Genetics (LGCM), Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
  • Costa MM; Laboratory of Microbiology and Animal Immunology, Federal University of Vale of São Francisco (Univasf), Petrolina, Pernambuco, Brazil - Center for Open Access Genomic Analysis (CALAnGO), Federal University of Vale of São Francisco (Univasf), Petrolina, Pernambuco, Brazil.
  • Broetto L; Multicentric Graduate Program in Biochemistry and Molecular Biology at SBBq (PMBqBM), Federal University of Alagoas, Brazil.
  • Nakazato L; Laboratory of Veterinary Microbiology and Molecular Biology, Veterinary Hospital of Federal University of Mato Grosso, Cuiabá, MT, Brazil.
  • Dutra V; Laboratory of Veterinary Microbiology and Molecular Biology, Veterinary Hospital of Federal University of Mato Grosso, Cuiabá, MT, Brazil.
J Infect Dev Ctries ; 18(5): 726-731, 2024 May 30.
Article em En | MEDLINE | ID: mdl-38865389
ABSTRACT

INTRODUCTION:

Serratia marcescens is an opportunistic pathogen found ubiquitously in the environment and associated with a wide range of nosocomial infections. This multidrug-resistant bacterium has been a cause of concern for hospitals and healthcare facilities due to its ability to spread rapidly and cause outbreaks. Next generation sequencing genotyping of bacterial isolates has proven to be a valuable tool for tracking the spread and transmission of nosocomial infections. This has allowed for the identification of outbreaks and transmission chains, as well as determining whether cases are due to endogenous or exogenous sources. Evidence of nosocomial transmission has been gathered through genotyping methods. The aim of this study was to investigate the genetic diversity of carbapenemase-producing S. marcescens in an outbreak at a public hospital in Cuiaba, MT, Brazil.

METHODOLOGY:

Ten isolates of S. marcenses were sequenced and antibiotic resistance profiles analyzed over 12 days.

RESULTS:

The isolates were clonal and multidrug resistant. Gentamycin and tigecycline had sensitivity in 90% and 80% isolates, respectively. Genomic analysis identified several genes that encode ß-lactamases, aminoglycoside-modifying enzymes, efflux pumps, and other virulence factors.

CONCLUSIONS:

Systematic surveillance is crucial in monitoring the evolution of S. marcescens genotypes, as it can lead to early detection and prevention of outbreaks.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Serratia marcescens / Infecção Hospitalar / Surtos de Doenças / Infecções por Serratia / Farmacorresistência Bacteriana Múltipla / Sequenciamento Completo do Genoma / Unidades de Terapia Intensiva / Antibacterianos Limite: Humans País/Região como assunto: America do sul / Brasil Idioma: En Revista: J Infect Dev Ctries Assunto da revista: DOENCAS TRANSMISSIVEIS Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Itália
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Serratia marcescens / Infecção Hospitalar / Surtos de Doenças / Infecções por Serratia / Farmacorresistência Bacteriana Múltipla / Sequenciamento Completo do Genoma / Unidades de Terapia Intensiva / Antibacterianos Limite: Humans País/Região como assunto: America do sul / Brasil Idioma: En Revista: J Infect Dev Ctries Assunto da revista: DOENCAS TRANSMISSIVEIS Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Itália