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Comparative evaluation of HPV genotyping: A study on the performance concordance between Anyplex II HPV28 detection and Linear Array genotyping tests in nationwide studies in Brazil.
Bandeira, Isabel Cristina; Comerlato, Juliana; Bessel, Marina; Fernandes, Bruna Vieira; Mota, Giana; Villa, Luisa Lina; de Souza, Flávia Moreno Alves; Pereira, Gerson Fernando Mendes; Wendland, Eliana Marcia.
Afiliação
  • Bandeira IC; Hospital Moinhos de Vento, Porto Alegre, Brazil.
  • Comerlato J; Hospital Moinhos de Vento, Porto Alegre, Brazil.
  • Bessel M; Hospital Moinhos de Vento, Porto Alegre, Brazil.
  • Fernandes BV; Hospital Moinhos de Vento, Porto Alegre, Brazil.
  • Mota G; Innovation in Cancer Laboratory, Center for Translational Research in Oncology of the Instituto do Câncer do Estado de São Paulo (ICESP), São Paulo, Brazil.
  • Villa LL; Innovation in Cancer Laboratory, Center for Translational Research in Oncology of the Instituto do Câncer do Estado de São Paulo (ICESP), São Paulo, Brazil.
  • de Souza FMA; Department of Chronic Conditions and Sexually Transmitted Infections, Ministry of Health, Brasília, Brazil.
  • Pereira GFM; Department of Chronic Conditions and Sexually Transmitted Infections, Ministry of Health, Brasília, Brazil.
  • Wendland EM; Hospital Moinhos de Vento, Porto Alegre, Brazil.
PLoS One ; 19(6): e0305122, 2024.
Article em En | MEDLINE | ID: mdl-38861542
ABSTRACT

BACKGROUND:

Advances in laboratory techniques for HPV diagnosis necessitate a thorough assessment of the efficiency, replicability, sensitivity, and specificity of those methods. This study aims to validate and compare HPV detection/genotyping using the Anyplex™ II HPV28 Detection assay (Seegene) assay and the Linear Array HPV Genotyping test (Roche Diagnostics) on genital samples for use in epidemiological studies.

METHODS:

From 6,388 penile and cervical DNA samples collected in the POP-Brazil, 1,745 were randomly selected to be included in this study. The samples were submitted to HPV detection and genotyping following the manufacturers' protocols. DNA was genotyped using the Anyplex™ II HPV28 Detection kit (Seegene), and the results were compared to those obtained using the Linear Array HPV Genotyping test (Roche Diagnostics). Concordance of HPV genotyping results was assessed by the percentage agreement and Cohen's kappa score (κ).

RESULTS:

The agreement between the two methodologies was deemed good for HPV detection (κ = 0.78). Notably, Anyplex™ II HPV28 demonstrated enhanced capability in detecting a broader spectrum of genotypes compared to Linear Array.

CONCLUSION:

Anyplex™ II HPV28 exhibited comparable results to the Linear Array assay in clinical specimens, showcasing its potential suitability for a diverse array of research applications requiring the detection and genotyping of HPV. The study supports the utility of Anyplex™ II HPV28 as an effective tool for HPV screening in epidemiological studies, emphasizing its robust performance in comparison to established diagnostic tests.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Papillomaviridae / Infecções por Papillomavirus / Técnicas de Genotipagem / Genótipo Limite: Adult / Female / Humans / Male / Middle aged País/Região como assunto: America do sul / Brasil Idioma: En Revista: PLoS One Assunto da revista: CIENCIA / MEDICINA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Papillomaviridae / Infecções por Papillomavirus / Técnicas de Genotipagem / Genótipo Limite: Adult / Female / Humans / Male / Middle aged País/Região como assunto: America do sul / Brasil Idioma: En Revista: PLoS One Assunto da revista: CIENCIA / MEDICINA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos