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Protocol for forward genetic screening to identify novel factors involved in a biological process in Caenorhabditis elegans.
Yoshida, Keita; Suehiro, Yuji; Mitani, Shohei.
Afiliação
  • Yoshida K; Department of Physiology, Tokyo Women's Medical University School of Medicine, 8-1 Kawada-cho, Shinjuku-ku, Tokyo 162-8666, Japan. Electronic address: yoshida.keita@twmu.ac.jp.
  • Suehiro Y; Department of Physiology, Tokyo Women's Medical University School of Medicine, 8-1 Kawada-cho, Shinjuku-ku, Tokyo 162-8666, Japan.
  • Mitani S; Department of Physiology, Tokyo Women's Medical University School of Medicine, 8-1 Kawada-cho, Shinjuku-ku, Tokyo 162-8666, Japan; Department of Physiology, The Nippon Dental University School of Life Dentistry, 1-9-20 Fujimi, Chiyoda-ku, Tokyo 102-8159, Japan. Electronic address: smitani514@gmail.com.
STAR Protoc ; 5(2): 103114, 2024 Jun 21.
Article em En | MEDLINE | ID: mdl-38843399
ABSTRACT
Forward genetic screens have been a powerful tool for discovering genes involved in various biological processes in Caenorhabditis elegans. Here, we present a protocol for forward genetic screening to identify novel factors involved in a biological process in C. elegans. We describe steps for mutagenesis, screening, and backcrossing. To save time and effort, we also detail procedures for utilizing whole-genome sequencing to exclude mutants of previously characterized genes from crosses for mapping mutations. For complete details on the use and execution of this protocol, please refer to Yoshida et al.1.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Testes Genéticos / Caenorhabditis elegans Limite: Animals Idioma: En Revista: STAR Protoc Ano de publicação: 2024 Tipo de documento: Article País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Testes Genéticos / Caenorhabditis elegans Limite: Animals Idioma: En Revista: STAR Protoc Ano de publicação: 2024 Tipo de documento: Article País de publicação: Estados Unidos