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Thermo-Alkali-Tolerant Recombinant Laccase from Bacillus swezeyi and Its Degradation Potential against Zearalenone and Aflatoxin B1.
Mwabulili, Fred; Xie, Yanli; Sun, Shumin; Ma, Weibin; Li, Qian; Yang, Yuhui; Jia, Hang; Li, Xiao.
Afiliação
  • Mwabulili F; College of Food Science and Engineering, Henan Key Laboratory of Cereal and Oil Food Safety and Nutrition, Henan University of Technology, Zhengzhou, Henan 450001, China.
  • Xie Y; Department of Food Science and Technology, College of Agricultural Sciences and Technology, Mbeya University of Science and Technology, P.O. Box 131, Mbeya 53119, Tanzania.
  • Sun S; College of Food Science and Engineering, Henan Key Laboratory of Cereal and Oil Food Safety and Nutrition, Henan University of Technology, Zhengzhou, Henan 450001, China.
  • Ma W; College of Food Science and Engineering, Henan Key Laboratory of Cereal and Oil Food Safety and Nutrition, Henan University of Technology, Zhengzhou, Henan 450001, China.
  • Li Q; College of Food Science and Engineering, Henan Key Laboratory of Cereal and Oil Food Safety and Nutrition, Henan University of Technology, Zhengzhou, Henan 450001, China.
  • Yang Y; College of Food Science and Engineering, Henan Key Laboratory of Cereal and Oil Food Safety and Nutrition, Henan University of Technology, Zhengzhou, Henan 450001, China.
  • Jia H; College of Food Science and Engineering, Henan Key Laboratory of Cereal and Oil Food Safety and Nutrition, Henan University of Technology, Zhengzhou, Henan 450001, China.
  • Li X; College of Food Science and Engineering, Henan Key Laboratory of Cereal and Oil Food Safety and Nutrition, Henan University of Technology, Zhengzhou, Henan 450001, China.
J Agric Food Chem ; 72(23): 13371-13381, 2024 Jun 12.
Article em En | MEDLINE | ID: mdl-38809574
ABSTRACT
The enzymatic biodegradation of mycotoxins in food and feed has attracted the most interest in recent years. In this paper, the laccase gene from Bacillus swezeyi was cloned and expressed in Escherichia coli BL 21(D3). The sequence analysis indicated that the gene consisted of 1533 bp. The purified B. swezeyi laccase was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis -12% with an estimated molecular weight of 56.7 kDa. The enzyme is thermo-alkali-tolerant, displaying the optimal degradation of zearalenone (ZEN) and aflatoxin B1 (AFB1) at pH 8 and 9, with incubation temperatures of 55 and 50 °C, respectively, within 24 h. The degradation potentials of the 50 µg of the enzyme against ZEN (5.0 µg/mL) and AFB1 (2.5 µg/mL) were 99.60 and 96.73%, respectively, within 24 h. To the best of our knowledge, this is the first study revealing the recombinant production of laccase from B. swezeyi, its biochemical properties, and potential use in ZEN and AFB1 degradation in vitro and in vivo.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Bacillus / Proteínas de Bactérias / Zearalenona / Estabilidade Enzimática / Proteínas Recombinantes / Aflatoxina B1 / Lacase Idioma: En Revista: J Agric Food Chem Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China País de publicação: Estados Unidos
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Bacillus / Proteínas de Bactérias / Zearalenona / Estabilidade Enzimática / Proteínas Recombinantes / Aflatoxina B1 / Lacase Idioma: En Revista: J Agric Food Chem Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China País de publicação: Estados Unidos