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Effect of transgenerational diabetes via maternal lineage in female rats.
Quintanilha Gallego, Franciane; Barco, Vinícius Soares; Sinzato, Yuri Karen; Paula, Verônyca Gonçalves; de Souza, Maysa Rocha; Lopes da Cruz, Larissa; Roy, Sayon; Corrente, José Eduardo; Damasceno, Débora Cristina.
Afiliação
  • Quintanilha Gallego F; Laboratory of Experimental Research on Gynecology and Obstetrics (UNIPEX), Course of Postgraduate on Tocogynecology, Botucatu Medical School, Sao Paulo State University (Unesp), Botucatu, São Paulo State, Brazil.
  • Barco VS; Laboratory of Experimental Research on Gynecology and Obstetrics (UNIPEX), Course of Postgraduate on Tocogynecology, Botucatu Medical School, Sao Paulo State University (Unesp), Botucatu, São Paulo State, Brazil.
  • Sinzato YK; Laboratory of Experimental Research on Gynecology and Obstetrics (UNIPEX), Course of Postgraduate on Tocogynecology, Botucatu Medical School, Sao Paulo State University (Unesp), Botucatu, São Paulo State, Brazil.
  • Paula VG; Laboratory of Experimental Research on Gynecology and Obstetrics (UNIPEX), Course of Postgraduate on Tocogynecology, Botucatu Medical School, Sao Paulo State University (Unesp), Botucatu, São Paulo State, Brazil.
  • de Souza MR; Laboratory of Experimental Research on Gynecology and Obstetrics (UNIPEX), Course of Postgraduate on Tocogynecology, Botucatu Medical School, Sao Paulo State University (Unesp), Botucatu, São Paulo State, Brazil.
  • Lopes da Cruz L; Laboratory of Experimental Research on Gynecology and Obstetrics (UNIPEX), Course of Postgraduate on Tocogynecology, Botucatu Medical School, Sao Paulo State University (Unesp), Botucatu, São Paulo State, Brazil.
  • Roy S; Department of Ophthalmology, School of Medicine, Boston University, Boston, MA, USA.
  • Corrente JE; Research Support Office, Botucatu Medical School, Sao Paulo State University (Unesp), Botucatu, São Paulo State, Brazil.
  • Damasceno DC; Laboratory of Experimental Research on Gynecology and Obstetrics (UNIPEX), Course of Postgraduate on Tocogynecology, Botucatu Medical School, Sao Paulo State University (Unesp), Botucatu, São Paulo State, Brazil.
Heliyon ; 10(10): e31049, 2024 May 30.
Article em En | MEDLINE | ID: mdl-38803977
ABSTRACT

Aim:

To investigate the transgenerational effect of maternal hyperglycemia on oxidative stress markers, lipid profile, glycemia, pancreatic beta (ß)-cells, and reproductive outcomes in the F2 adult generation. Additionally, to expand the knowledge on transgenerational diabetes the F3 generation at birth will be evaluated.

Methods:

On day 5 of postnatal life female Sprague-Dawley rat newborns (F0 generation) were distributed into two groups Diabetic (Streptozotocin-STZ, 70 mg/kg body weight, subcutaneous route) and Control rats. Adult female rats from the F0 generation and subsequently the F1 generation were mated to obtain the F2 generation, which was distributed into F2 generation (granddaughters) from control (F2_C) and diabetic (F2_D) rats. Oral Glucose Tolerance Test (OGTT), the area under the curve (AUC), blood biochemical analyses, and pancreatic morphology were analyzed before pregnancy. Reproductive outcomes were performed at the end of pregnancy. At birth, the glycemia and body weight of F3_C and F3_D rats were determined. p < 0.05 was considered significant.

Results:

F2_D had higher body weight, triglyceride levels, and percentage of insulin-immunostained cells, contributing to glucose intolerance, and insulin resistance before pregnancy. At day 21 of pregnancy, the F2_D showed increased embryonic losses before and after implantation (84.33 and 83.74 %, respectively). At birth, F3_D presented hyperglycemia, and 16.3 % of newborns were large for pregnancy age (LGA).

Conclusion:

Diabetes induction since the neonatal period in the first generation (F0) led to transgenerational (F2 and F3 generations) changes via the maternal lineage of female rats, confirming the relevance of control strictly the glycemia all the time.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Heliyon Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Heliyon Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido