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Microwave-Assisted Extraction of Secondary Metabolites Using Ethyl Lactate Green Solvent from Ambrosia arborescens: LC/ESI-MS/MS and Antioxidant Activity.
Guillen, Evelyn; Terrones, Hector; de Terrones, Teresa Cano; Simirgiotis, Mario J; Hájek, Jan; Cheel, José; Sepulveda, Beatriz; Areche, Carlos.
Afiliação
  • Guillen E; Departamento de Química, Facultad de Ciencias Naturales y Formales, Universidad Nacional de San Agustín, Arequipa 68513, Peru.
  • Terrones H; Departamento de Química, Facultad de Ciencias Naturales y Formales, Universidad Nacional de San Agustín, Arequipa 68513, Peru.
  • de Terrones TC; Departamento de Química, Facultad de Ciencias Naturales y Formales, Universidad Nacional de San Agustín, Arequipa 68513, Peru.
  • Simirgiotis MJ; Instituto de Farmacia, Facultad de Ciencias, Campus Isla Teja, Universidad Austral de Chile, Valdivia 5090000, Chile.
  • Hájek J; Laboratory of Algal Biotechnology, Centre ALGATECH, Institute of Microbiology of the Czech Academy of Sciences, Opatovický Mlýn, 379 81 Trebon, Czech Republic.
  • Cheel J; Laboratory of Algal Biotechnology, Centre ALGATECH, Institute of Microbiology of the Czech Academy of Sciences, Opatovický Mlýn, 379 81 Trebon, Czech Republic.
  • Sepulveda B; Departamento de Ciencias Químicas, Universidad Andrés Bello, Campus Viña del Mar, Quillota 980, Viña del Mar 2520000, Chile.
  • Areche C; Departamento de Química, Facultad de Ciencias, Universidad de Chile, Las Palmeras 3425, Nuñoa, Santiago 8320000, Chile.
Plants (Basel) ; 13(9)2024 Apr 27.
Article em En | MEDLINE | ID: mdl-38732429
ABSTRACT
Alternative solvents are being tested as green solvents to replace the traditional organic solvents used in both academy and industry. Some of these are already available, such as ethyl lactate, cyrene, limonene, glycerol, and others. This alternative explores eco-friendly processes for extracting secondary metabolites from nature, thus increasing the number of unconventional extraction methods with lower environmental impact over conventional methods. In this context, the Peruvian Ambrosia arborescens was our model while exploring a microwave-assisted extraction (MAE) approach over maceration. The objective of this study was to perform a phytochemical study including UHPLC-ESI-MS/MS and the antioxidant activity of Ambrosia arborescens, using sustainable strategies by mixing both microwaves and ethyl lactate as a green solvent. The results showed that ethyl lactate/MAE (15.07%) achieved a higher extraction yield than methanol/maceration (12.6%). In the case of the isolation of psilostachyin, it was similar to ethyl lactate (0.44%) when compared to methanol (0.40%). Regarding UHPLC-ESI-MS/MS studies, the results were similar. Twenty-eight compounds were identified in the ethyl lactate/MAE and methanol/maceration extracts, except for the tentative identification of two additional amino acids (peaks 4 and 6) in the MeOH extract. In relation to the antioxidant assay, the activity of the ethyl lactate extract was a little higher than the methanol extract in terms of ORAC (715.38 ± 3.2) and DPPH (263.04 ± 2.8). This study on A. arborescens demonstrated that the unconventional techniques, such as MAE related to ethyl lactate, could replace maceration/MeOH for the extraction and isolation of metabolites from diverse sources. This finding showed the potential of unconventional methods with green solvents to provide eco-friendly methods based on green chemistry.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Plants (Basel) Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Peru País de publicação: Suíça

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Plants (Basel) Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Peru País de publicação: Suíça