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Mild hyperhomocysteinemia alters oxidative stress profile via Nrf2, inflammation and cholinesterases in cardiovascular system of aged male rats.
Prauchner, Gustavo Ricardo Krupp; Ramires Junior, Osmar Vieira; Rieder, Alessandra Schmitt; Wyse, Angela T S.
Afiliação
  • Prauchner GRK; Laboratory of Neuroprotection and Neurometabolic Diseases, Department of Biochemistry, Wyse's Lab, ICBS, Universidade Federal do Rio Grande do Sul (UFRGS), Rua Ramiro Barcelos, 2600-Anexo, 90035-003, Porto Alegre, RS, Brazil.
  • Ramires Junior OV; Laboratory of Neuroprotection and Neurometabolic Diseases, Department of Biochemistry, Wyse's Lab, ICBS, Universidade Federal do Rio Grande do Sul (UFRGS), Rua Ramiro Barcelos, 2600-Anexo, 90035-003, Porto Alegre, RS, Brazil.
  • Rieder AS; Laboratory of Neuroprotection and Neurometabolic Diseases, Department of Biochemistry, Wyse's Lab, ICBS, Universidade Federal do Rio Grande do Sul (UFRGS), Rua Ramiro Barcelos, 2600-Anexo, 90035-003, Porto Alegre, RS, Brazil.
  • Wyse ATS; Laboratory of Neuroprotection and Neurometabolic Diseases, Department of Biochemistry, Wyse's Lab, ICBS, Universidade Federal do Rio Grande do Sul (UFRGS), Rua Ramiro Barcelos, 2600-Anexo, 90035-003, Porto Alegre, RS, Brazil. Electronic address: wyse@ufrgs.br.
Chem Biol Interact ; 396: 111028, 2024 Jun 01.
Article em En | MEDLINE | ID: mdl-38729282
ABSTRACT
Homocysteine (Hcy) is an independent cardiovascular disease (CVD) risk factor, whose mechanisms are poorly understood. We aimed to explore mild hyperhomocysteinemia (HHcy) effects on oxidative status, inflammatory, and cholinesterase parameters in aged male Wistar rats (365 days old). Rats received subcutaneous Hcy (0.03 µmol/g body weight) twice daily for 30 days, followed by euthanasia, blood collection and heart dissection 12 h after the last injection. Results revealed increased dichlorofluorescein (DCF) levels in the heart and serum, alongside decreased antioxidant enzyme activities (superoxide dismutase, catalase, glutathione peroxidase), reduced glutathione (GSH) content, and diminished acetylcholinesterase (AChE) activity in the heart. Serum butyrylcholinesterase (BuChE) levels also decreased. Furthermore, nuclear factor erythroid 2-related factor 2 (Nrf2) protein content decreased in both cytosolic and nuclear fractions, while cytosolic nuclear factor kappa B (NFκB) p65 increased in the heart. Additionally, interleukins IL-1ß, IL-6 and IL-10 showed elevated expression levels in the heart. These findings could suggest a connection between aging and HHcy in CVD. Reduced Nrf2 protein content and impaired antioxidant defenses, combined with inflammatory factors and altered cholinesterases activity, may contribute to understanding the impact of Hcy on cardiovascular dynamics. This study sheds light on the complex interplay between HHcy, oxidative stress, inflammation, and cholinesterases in CVD, providing valuable insights for future research.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ratos Wistar / Estresse Oxidativo / Hiper-Homocisteinemia / Fator 2 Relacionado a NF-E2 / Inflamação Limite: Animals Idioma: En Revista: Chem Biol Interact Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Irlanda
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ratos Wistar / Estresse Oxidativo / Hiper-Homocisteinemia / Fator 2 Relacionado a NF-E2 / Inflamação Limite: Animals Idioma: En Revista: Chem Biol Interact Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Irlanda