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Muscle fibre morphometric analysis (MusMA) correlates with muscle function and cardiovascular risk prognosis.
Longo, Larisse; Bartikoski, Bárbara Jonson; de Souza, Valessa Emanoele Gabriel; Salvati, Fernando; Uribe-Cruz, Carolina; Lenz, Guido; Xavier, Ricardo Machado; Álvares-da-Silva, Mário Reis; Filippi-Chiela, Eduardo Cremonese.
Afiliação
  • Longo L; Graduate Program in Gastroenterology and Hepatology, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
  • Bartikoski BJ; Experimental Hepatology and Gastroenterology Laboratory, Center for Experimental Research, Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil.
  • de Souza VEG; Autoimmune Diseases Laboratory, Rheumatology Service, Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil.
  • Salvati F; Experimental Hepatology and Gastroenterology Laboratory, Center for Experimental Research, Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil.
  • Uribe-Cruz C; Experimental Hepatology and Gastroenterology Laboratory, Center for Experimental Research, Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil.
  • Lenz G; Graduate Program in Gastroenterology and Hepatology, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
  • Xavier RM; Experimental Hepatology and Gastroenterology Laboratory, Center for Experimental Research, Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil.
  • Álvares-da-Silva MR; Universidad Católica de las Misiones, Posadas, Argentina.
  • Filippi-Chiela EC; Department of Biophysics and Biotechnology Center, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
Int J Exp Pathol ; 105(3): 100-113, 2024 Jun.
Article em En | MEDLINE | ID: mdl-38722178
ABSTRACT
Morphometry of striated muscle fibres is critical for monitoring muscle health and function. Here, we evaluated functional parameters of skeletal and cardiac striated muscle in two experimental models using the Morphometric Analysis of Muscle Fibre tool (MusMA). The collagen-induced arthritis model was used to evaluate the function of skeletal striated muscle and the non-alcoholic fatty liver disease model was used for cardiac striated muscle analysis. After euthanasia, we used haeamatoxylin and eosin stained sections of skeletal and cardiac muscle to perform muscle fibre segmentation and morphometric analysis. Morphometric analysis classified muscle fibres into six subpopulations normal, regular hypertrophic, irregular hypertrophic, irregular, irregular atrophic and regular atrophic. The percentage of atrophic fibres was associated with lower walking speed (p = 0.009) and lower body weight (p = 0.026), respectively. Fibres categorized as normal were associated with maximum grip strength (p < 0.001) and higher march speed (p < 0.001). In the evaluation of cardiac striated muscle fibres, the percentage of normal cardiomyocytes negatively correlated with cardiovascular risk markers such as the presence of abdominal adipose tissue (p = .003), miR-33a expression (p = .001) and the expression of miR-126 (p = .042) Furthermore, the percentage of atrophic cardiomyocytes correlated significantly with the Castelli risk index II (p = .014). MusMA is a simple and objective tool that allows the screening of striated muscle fibre morphometry, which can complement the diagnosis of muscle diseases while providing functional and prognostic information in basic and clinical research.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fibras Musculares Esqueléticas Limite: Animals Idioma: En Revista: Int J Exp Pathol Assunto da revista: PATOLOGIA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fibras Musculares Esqueléticas Limite: Animals Idioma: En Revista: Int J Exp Pathol Assunto da revista: PATOLOGIA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido