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Diagnostic performance of the bioline dengue duo rapid test on symptomatic patients assisted at Armed Forces Hospital (Hfa) in Brasília, Brazil.
Gomes, Leani Falcão; Mundim, Júlia Viegas; do Carmo Silva, Lívia; de Curcio, Juliana Santana; de Sousa, Adriano Roberto Vieira; Garcia-Zapata, Marco Tulio; Anunciação, Carlos Eduardo; do Carmo, Juliana Ramos; de Paula Silveira-Lacerda, Elisângela.
Afiliação
  • Gomes LF; Department of Genetics, Federal University of Goias, Avenida Esperança, s/n, Câmpus Samambaia, Goiania, GO, 74.690-900, Brazil.
  • Mundim JV; Research Division of Technical Teaching and Research Directorate, Armed Forces Hospital, Brasilia, DF, Brazil.
  • do Carmo Silva L; Research Division of Technical Teaching and Research Directorate, Armed Forces Hospital, Brasilia, DF, Brazil.
  • de Curcio JS; Department of Genetics, Federal University of Goias, Avenida Esperança, s/n, Câmpus Samambaia, Goiania, GO, 74.690-900, Brazil.
  • de Sousa ARV; Department of Genetics, Federal University of Goias, Avenida Esperança, s/n, Câmpus Samambaia, Goiania, GO, 74.690-900, Brazil.
  • Garcia-Zapata MT; Department of Genetics, Federal University of Goias, Avenida Esperança, s/n, Câmpus Samambaia, Goiania, GO, 74.690-900, Brazil.
  • Anunciação CE; Department of Tropical Medicine and Dermatology, Federal University of Goias, Goiania, GO, Brazil.
  • do Carmo JR; Department of Biochemistry and Molecular Biology, Federal University of Goias, Goiania, GO, Brazil.
  • de Paula Silveira-Lacerda E; Department of Genetics, Federal University of Goias, Avenida Esperança, s/n, Câmpus Samambaia, Goiania, GO, 74.690-900, Brazil.
Braz J Microbiol ; 55(2): 1801-1809, 2024 Jun.
Article em En | MEDLINE | ID: mdl-38709438
ABSTRACT
Dengue necessitates accurate diagnosis. Rapid tests such as Bioline™ DENGUE DUO have gained traction, but validation in specific populations is essential. This study aimed to evaluate the performance of the Bioline™ test, alongside assessing the socio-epidemiological profile of symptomatic patients in a Brasília Military Hospital. The serum of 404 symptomatic patients was analyzed by the Bioline™ DENGUE DUO test, followed by Dengue virus detection and discrimination of the four serotypes by RT-qPCR. Accuracy was assessed using parameters including sensitivity (S), specificity (E), positive and negative predictive values (PPV and NPV), and positive (RV +) and negative (RV-) likelihood ratios. The NS1 component exhibited a sensitivity of 70.37%, a specificity of 97.30%, and an overall efficiency of 90.10% when compared to RT-qPCR as the gold standard. The IgM component demonstrated a sensitivity of 26.85%, a specificity of 89.53%, and an overall efficiency of 72.77% when compared to RT-qPCR as the gold standard. The IgG component demonstrated a sensitivity of 23.15%, a specificity of 68.92%, and an overall efficiency of 56.68% when compared to RT-qPCR as the gold standard. Several rapid tests are commercially available. However, considering variations across regions and demographic groups, it is important to question their accuracy in specific populations. Rapid tests are important screening tools, but they can have limitations for the certainty of diagnosis. Bioline™ DENGUE DUO displayed good specificity, but sensitivity was slightly below optimal levels. While helpful for confirming dengue, improvements are needed to effectively rule out the disease.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Sensibilidade e Especificidade / Dengue / Vírus da Dengue / Hospitais Militares Limite: Adolescent / Adult / Aged / Child / Child, preschool / Female / Humans / Male / Middle aged País/Região como assunto: America do sul / Brasil Idioma: En Revista: Braz J Microbiol Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Sensibilidade e Especificidade / Dengue / Vírus da Dengue / Hospitais Militares Limite: Adolescent / Adult / Aged / Child / Child, preschool / Female / Humans / Male / Middle aged País/Região como assunto: America do sul / Brasil Idioma: En Revista: Braz J Microbiol Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil