Your browser doesn't support javascript.
loading
Characterization of microsatellite markers in the coding regions of the Penaeus vannamei genome.
Mangabeira-Silva, Iasmim Santos; Soares, Paulo Eduardo Toscano; Vieira da Silva, Yago Tomaz; Rodrigues de Albuquerque, Beatriz Helena Dantas; Câmera de Oliveira, Maryana Thalyta Ferreira; Ferreira, Larissa Alves Honorato; Bezerra de Souza, Maria Fernanda; Vieira de Lucena, Danyllo; Paiva Pereira, Jessica Marina; Pinheiro E Silva, Roseli Pimentel; Lanza, Daniel Carlos Ferreira.
Afiliação
  • Mangabeira-Silva IS; Laboratory of Applied Molecular Biology-LAPLIC, Federal University of Rio Grande do Norte, Natal, RN, Brazil.
  • Soares PET; Postgraduate Program in Biotechnology, RENORBIO, Natal, RN, Brazil.
  • Vieira da Silva YT; Laboratory of Applied Molecular Biology-LAPLIC, Federal University of Rio Grande do Norte, Natal, RN, Brazil.
  • Rodrigues de Albuquerque BHD; Postgraduate Program in Biochemistry, Federal University of Rio Grande do Norte, Natal, RN, Brazil.
  • Câmera de Oliveira MTF; Laboratory of Applied Molecular Biology-LAPLIC, Federal University of Rio Grande do Norte, Natal, RN, Brazil.
  • Ferreira LAH; Laboratory of Applied Molecular Biology-LAPLIC, Federal University of Rio Grande do Norte, Natal, RN, Brazil.
  • Bezerra de Souza MF; Postgraduate Program in Biochemistry, Federal University of Rio Grande do Norte, Natal, RN, Brazil.
  • Vieira de Lucena D; Laboratory of Applied Molecular Biology-LAPLIC, Federal University of Rio Grande do Norte, Natal, RN, Brazil.
  • Paiva Pereira JM; Postgraduate Program in Biochemistry, Federal University of Rio Grande do Norte, Natal, RN, Brazil.
  • Pinheiro E Silva RP; Laboratory of Applied Molecular Biology-LAPLIC, Federal University of Rio Grande do Norte, Natal, RN, Brazil.
  • Lanza DCF; Laboratory of Applied Molecular Biology-LAPLIC, Federal University of Rio Grande do Norte, Natal, RN, Brazil.
PLoS One ; 19(5): e0289351, 2024.
Article em En | MEDLINE | ID: mdl-38696386
ABSTRACT
In this study, an extensive analysis of microsatellite markers (Single Tandem Repeats-STRs) in Penaeus vannamei was conducted at an advanced level. The markers were thoroughly examined, characterized, and specific markers located within coding regions were identified. Out of a total of 306 STRs, 117 were classified as perfect markers based on their single repeat motif. Among these perfect markers, 62 were found to be associated with predicted coding genes (mRNA), which were involved in various functions such as binding, catalytic activity, ATP-dependent activity, transcription, structural and molecular regulation. To validate the accuracy of the findings, a sample of nine markers was subjected to in vitro testing, which confirmed the presence of polymorphisms within the population. These results suggest the existence of different protein isoforms within the population, indicating the potential of these markers for application in both population and phenotype-genotype association studies. This innovative approach opens up new possibilities for investigating the impact of genomic plasticity in populations of P. vannamei.
Assuntos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Repetições de Microssatélites / Penaeidae Limite: Animals Idioma: En Revista: PLoS One Assunto da revista: CIENCIA / MEDICINA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Repetições de Microssatélites / Penaeidae Limite: Animals Idioma: En Revista: PLoS One Assunto da revista: CIENCIA / MEDICINA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos