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Obesity aggravates acute kidney injury resulting from ischemia and reperfusion in mice.
da Silva, Igor Oliveira; de Menezes, Nicole K; Jacobina, Heloisa D; Parra, Antonio Carlos; Souza, Felipe Lima; Castro, Leticia Cardoso; Roelofs, Joris J T H; Tammaro, Alessandra; Gomes, Samirah Abreu; Sanches, Talita Rojas; Andrade, Lucia.
Afiliação
  • da Silva IO; Laboratory of Basic Science in Renal Diseases (LIM-12), Division of Nephrology, University of São Paulo School of Medicine, São Paulo, Brazil.
  • de Menezes NK; Laboratory of Basic Science in Renal Diseases (LIM-12), Division of Nephrology, University of São Paulo School of Medicine, São Paulo, Brazil.
  • Jacobina HD; Laboratory of Basic Science in Renal Diseases (LIM-12), Division of Nephrology, University of São Paulo School of Medicine, São Paulo, Brazil.
  • Parra AC; Laboratory of Basic Science in Renal Diseases (LIM-12), Division of Nephrology, University of São Paulo School of Medicine, São Paulo, Brazil.
  • Souza FL; Laboratory of Cellular Genetic and Molecular Nephrology, Division of Nephrology, Av. Dr. Arnaldo, 455, 3º Andar, sala 3310, University of São Paulo School of Medicine, São Paulo, SP, CEP 01246-903, Brazil.
  • Castro LC; Laboratory of Basic Science in Renal Diseases (LIM-12), Division of Nephrology, University of São Paulo School of Medicine, São Paulo, Brazil.
  • Roelofs JJTH; Department of Pathology, Amsterdam UMC, Location AMC, University of Amsterdam, Amsterdam, the Netherlands.
  • Tammaro A; Amsterdam Cardiovascular Sciences, University of Amsterdam, Amsterdam, the Netherlands.
  • Gomes SA; Department of Pathology, Amsterdam UMC, Location AMC, University of Amsterdam, Amsterdam, the Netherlands.
  • Sanches TR; Laboratory of Cellular Genetic and Molecular Nephrology, Division of Nephrology, Av. Dr. Arnaldo, 455, 3º Andar, sala 3310, University of São Paulo School of Medicine, São Paulo, SP, CEP 01246-903, Brazil.
  • Andrade L; Laboratory of Basic Science in Renal Diseases (LIM-12), Division of Nephrology, University of São Paulo School of Medicine, São Paulo, Brazil.
Sci Rep ; 14(1): 9820, 2024 04 29.
Article em En | MEDLINE | ID: mdl-38684767
ABSTRACT
In critically ill patients, overweight and obesity are associated with acute respiratory distress syndrome and acute kidney injury (AKI). However, the effect of obesity on ischemia-reperfusion injury (IRI)-induced AKI is unknown. We hypothesized that obesity would aggravate renal IRI in mice. We fed mice a standard or high-fat diet for eight weeks. The mice were divided into four groups and submitted to sham surgery or IRI obese, normal, normal + IRI, obese, and obese + IRI. All studies were performed 48 h after the procedures. Serum glucose, cholesterol, and creatinine clearance did not differ among the groups. Survival and urinary osmolality were lower in the obese + IRI group than in the normal + IRI group, whereas urinary neutrophil gelatinase-associated lipocalin levels, tubular injury scores, and caspase 3 expression were higher. Proliferating cell nuclear antigen expression was highest in the obese + IRI group, as were the levels of oxidative stress (urinary levels of thiobarbituric acid-reactive substances and renal heme oxygenase-1 protein expression), whereas renal Klotho protein expression was lowest in that group. Expression of glutathione peroxidase 4 and peroxiredoxin 6, proteins that induce lipid peroxidation, a hallmark of ferroptosis, was lower in the obese + IRI group. Notably, among the mice not induced to AKI, macrophage infiltration was greater in the obese group. In conclusion, greater oxidative stress and ferroptosis might aggravate IRI in obese individuals, and Klotho could be a therapeutic target in those with AKI.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Traumatismo por Reperfusão / Estresse Oxidativo / Injúria Renal Aguda / Obesidade Limite: Animals Idioma: En Revista: Sci Rep Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Traumatismo por Reperfusão / Estresse Oxidativo / Injúria Renal Aguda / Obesidade Limite: Animals Idioma: En Revista: Sci Rep Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido