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Saturated Iso-Type Fatty Acids from the Marine Bacterium Mesoflavibacter zeaxanthinifaciens with Anti-Trypanosomal Potential.
Santos Ferreira, Dayana Agnes; de Castro Levatti, Erica Valadares; Santa Cruz, Lucas Monteiro; Costa, Alan Roberto; Migotto, Álvaro E; Yamada, Amanda Yaeko; Camargo, Carlos Henrique; Christodoulides, Myron; Lago, João Henrique G; Tempone, Andre Gustavo.
Afiliação
  • Santos Ferreira DA; Pathophysiology Laboratory, Instituto Butantan, Av. Vital Brazil, 1500, Sao Paulo 05503-900, SP, Brazil.
  • de Castro Levatti EV; Pathophysiology Laboratory, Instituto Butantan, Av. Vital Brazil, 1500, Sao Paulo 05503-900, SP, Brazil.
  • Santa Cruz LM; Centre of Organic Contaminants, Instituto Adolfo Lutz, Av. Dr. Arnaldo, 355, Sao Paulo 01246-000, SP, Brazil.
  • Costa AR; Centre of Organic Contaminants, Instituto Adolfo Lutz, Av. Dr. Arnaldo, 355, Sao Paulo 01246-000, SP, Brazil.
  • Migotto ÁE; Centre for Marine Biology, Universidade de São Paulo, Rodovia Doutor Manoel Hipólito do Rego, km. 131,5, Pitangueiras, Sao Sebastiao 11612-109, SP, Brazil.
  • Yamada AY; Centre of Bacteriology, Instituto Adolfo Lutz, Av. Dr. Arnaldo, 351, Sao Paulo 01246-000, SP, Brazil.
  • Camargo CH; Centre of Bacteriology, Instituto Adolfo Lutz, Av. Dr. Arnaldo, 351, Sao Paulo 01246-000, SP, Brazil.
  • Christodoulides M; Molecular Microbiology, School of Clinical and Experimental Sciences, Faculty of Medicine, University of Southampton, Southampton SO16 6YD, UK.
  • Lago JHG; Centre of Natural Sciences and Humanities, Universidade Federal do ABC, Sao Paulo 09210-580, SP, Brazil.
  • Tempone AG; Pathophysiology Laboratory, Instituto Butantan, Av. Vital Brazil, 1500, Sao Paulo 05503-900, SP, Brazil.
Pharmaceuticals (Basel) ; 17(4)2024 Apr 13.
Article em En | MEDLINE | ID: mdl-38675459
ABSTRACT
Chagas disease is a Neglected Tropical Disease with limited and ineffective therapy. In a search for new anti-trypanosomal compounds, we investigated the potential of the metabolites from the bacteria living in the corals and sediments of the southeastern Brazilian coast. Three corals, Tubastraea coccinea, Mussismilia hispida, Madracis decactis, and sediments yielded 11 bacterial strains that were fully identified by MALDI-ToF/MS or gene sequencing, resulting in six genera-Vibrio, Shewanella, Mesoflavibacter, Halomonas, Bacillus, and Alteromonas. To conduct this study, EtOAc extracts were prepared and tested against Trypanosoma cruzi. The crude extracts showed IC50 values ranging from 15 to 51 µg/mL against the trypomastigotes. The bacterium Mesoflavibacter zeaxanthinifaciens was selected for fractionation, resulting in an active fraction (FII) with IC50 values of 17.7 µg/mL and 23.8 µg/mL against the trypomastigotes and amastigotes, respectively, with neither mammalian cytotoxicity nor hemolytic activity. Using an NMR and ESI-HRMS analysis, the FII revealed the presence of unsaturated iso-type fatty acids. Its lethal action was investigated, leading to a protein spectral profile of the parasite altered after treatment. The FII also induced a rapid permeabilization of the plasma membrane of the parasite, leading to cell death. These findings demonstrate that these unsaturated iso-type fatty acids are possible new hits against T. cruzi.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Pharmaceuticals (Basel) Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Suíça
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Pharmaceuticals (Basel) Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Suíça