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Cefepime distribution by microdialysis in peritoneal fluid of rats with or without experimental peritonitis.
Dos Anjos, Michele Vaz; Possa, Eduarda; Fonseca, Gisele da Silva; Bergoza, Larissa; Tasso, Leandro.
Afiliação
  • Dos Anjos MV; Health Sciences Postgraduate Program, University of Caxias do Sul, Caxias do Sul, Brazil.
  • Possa E; Health Sciences Postgraduate Program, University of Caxias do Sul, Caxias do Sul, Brazil.
  • Fonseca GDS; Biotechnology Postgraduate Program, University of Caxias do Sul, Caxias do Sul, Brazil.
  • Bergoza L; Health Sciences Postgraduate Program, University of Caxias do Sul, Caxias do Sul, Brazil.
  • Tasso L; Health Sciences Postgraduate Program, University of Caxias do Sul, Caxias do Sul, Brazil.
APMIS ; 2024 Apr 24.
Article em En | MEDLINE | ID: mdl-38659357
ABSTRACT
The aim of this study was to investigate the penetration of cefepime into rat peritoneal fluid by microdialysis and to determine the relationship between unbound drug plasma and tissue concentration in healthy animals and in a sepsis model established through cecal ligation and puncture-induced peritonitis. Probe recovery was performed by dialysis and retrodialysis. Cefepime was administered at a dose of 110 mg/kg intravenously. Samples were collected for about 4 h, and concentrations were determined by liquid chromatography-electrospray ionization-QTOF MS. Tissue penetration was also determined. Probe recovery in vivo was 38.78% ± 3.31% and 38.83% ± 2.74% in the control and peritonitis groups, respectively. Cefepime was rapidly distributed in the peritoneal fluid in both groups. The peritoneal fluid/plasma cefepime ratio was 0.38 and 0.32 for the control and peritonitis groups, respectively. Cefepime concentrations were above the MIC of 4 mg/L for the main enterobacteria. The infection model that was used had no apparent effect on the pharmacokinetics of cefepime in rats. This was the first study to determine free cefepime concentrations in the peritoneal fluid of healthy rats and rats with experimental peritonitis.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: APMIS Assunto da revista: ALERGIA E IMUNOLOGIA / MICROBIOLOGIA / PATOLOGIA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Dinamarca
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: APMIS Assunto da revista: ALERGIA E IMUNOLOGIA / MICROBIOLOGIA / PATOLOGIA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Dinamarca