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Chemical control of CRISPR/Cpf1 editing via orthogonal activation and deactivation of crosslinked crRNA.
Lin, Cui-Lian; Chen, Wen-Da; Liu, Li; Cheng, Liang.
Afiliação
  • Lin CL; Beijing National Laboratory for Molecular Sciences (BNLMS), CAS Key Laboratory of Molecular Recognition and Function, CAS Research/Education Center for Excellence in Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China. chengl@iccas.ac.cn.
  • Chen WD; University of Chinese Academy of Sciences, Beijing 100049, China.
  • Liu L; Beijing National Laboratory for Molecular Sciences (BNLMS), CAS Key Laboratory of Molecular Recognition and Function, CAS Research/Education Center for Excellence in Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China. chengl@iccas.ac.cn.
  • Cheng L; University of Chinese Academy of Sciences, Beijing 100049, China.
Chem Commun (Camb) ; 60(39): 5197-5200, 2024 May 09.
Article em En | MEDLINE | ID: mdl-38651297
ABSTRACT
Through the integration of CRISPR/Cpf1 with optogenetics and a reduction-responsive motif, we have developed a photoactivatable cross-linked crRNA that enables precise genome editing upon light exposure. This system also allows for termination of editing activity through external application of reducing agent. The dual-stimuli-responsive CRISPR/Cpf1 editing process operates in a unique OFF → ON → OFF sequence, making it a valuable tool for investigating time-sensitive biological events.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Sistemas CRISPR-Cas / Edição de Genes Limite: Humans Idioma: En Revista: Chem Commun (Camb) Assunto da revista: QUIMICA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Sistemas CRISPR-Cas / Edição de Genes Limite: Humans Idioma: En Revista: Chem Commun (Camb) Assunto da revista: QUIMICA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China País de publicação: Reino Unido