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Protocol for the selection of Mycobacterium tuberculosis spontaneous resistant mutants to d-cycloserine.
Osorio-González, Alejandra; Álvarez, Nataly; Realpe, Teresa; Robledo, Jaime.
Afiliação
  • Osorio-González A; Unidad de Bacteriología y Micobacterias. Corporación para Investigaciones Biológicas, Medellín, Colombia.
  • Álvarez N; Escuela de Ciencias de la Salud. Universidad Pontificia Bolivariana, Medellín, Colombia.
  • Realpe T; Facultad de Ciencias Exactas y Naturales, Instituto de Biología (Maestría en biología), Universidad de Antioquia, Medellín, Colombia.
  • Robledo J; Unidad de Bacteriología y Micobacterias. Corporación para Investigaciones Biológicas, Medellín, Colombia.
MethodsX ; 12: 102690, 2024 Jun.
Article em En | MEDLINE | ID: mdl-38638452
ABSTRACT
Mycobacterium tuberculosis (MTB) is known for its adaptive capability in developing resistance to antibiotics, through the selection of spontaneous mutations that arise during treatment. Generating spontaneous antibiotic-resistant mutants in vitro is challenging but necessary for studying this phenomenon. A protocol was designed and tested to select stable, MTB spontaneous, d-cycloserine (DCS) resistant mutants. Twenty-four colonies resistant to DCS were selected, demonstrating an increase between 1 and 4 times the Minimum Inhibitory Concentration (MIC) set for Mycobacterium tuberculosis H37Rv ATCC 27294 reference strain.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: MethodsX Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Colômbia País de publicação: Holanda
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: MethodsX Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Colômbia País de publicação: Holanda