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Sens-ocular model: Cell-based assay to evaluate eye stinging potential of chemicals and baby cosmetic formulations.
Brito, Lara Barroso; Catarino, Carolina Motter; Passos, Maria Claudia; Garcia Silva, Artur C; Schuck, Desiree Cigaran; Canavez, Andrezza Di Pietro Micali; Valadares, Marize Campos.
Afiliação
  • Brito LB; Laboratório de Ensino e Pesquisa em Toxicologia In Vitro - Tox In, Faculdade de Farmácia, Universidade Federal de Goiás, Goiânia, GO, Brazil.
  • Catarino CM; Grupo Boticário, São José dos Pinhais, Curitiba, PR, Brazil.
  • Passos MC; Laboratório de Ensino e Pesquisa em Toxicologia In Vitro - Tox In, Faculdade de Farmácia, Universidade Federal de Goiás, Goiânia, GO, Brazil.
  • Garcia Silva AC; Laboratório de Ensino e Pesquisa em Toxicologia In Vitro - Tox In, Faculdade de Farmácia, Universidade Federal de Goiás, Goiânia, GO, Brazil.
  • Schuck DC; Grupo Boticário, São José dos Pinhais, Curitiba, PR, Brazil.
  • Canavez ADPM; Grupo Boticário, São José dos Pinhais, Curitiba, PR, Brazil.
  • Valadares MC; Laboratório de Ensino e Pesquisa em Toxicologia In Vitro - Tox In, Faculdade de Farmácia, Universidade Federal de Goiás, Goiânia, GO, Brazil. Electronic address: marizecv@ufg.br.
Toxicol In Vitro ; 98: 105824, 2024 Jun.
Article em En | MEDLINE | ID: mdl-38614139
ABSTRACT
The TRPV1 receptor, which is known to contribute significantly to pain perception, has recently been identified as a useful tool for predicting eye stinging potential in cosmetics. In this study, HEK-293 cells with high TRPV1 expression were utilized to evaluate calcium influx related to receptor activation triggered by chemicals and cosmetic formulations. The cells were exposed to increasing concentrations of substances to cause or not some aggression to the eye, and TRPV1 activity was assessed by measuring intracellular FURA-2 AM fluorescence signal. To confirm TRPV1 channel activation, capsazepine, a capsaicin antagonist, was employed in addition to using capsaicin as a positive control. The study's results indicate that this novel model can identify compounds known to cause some aggression to the eye, such as stinging, considering a cut-off value of 60% of Ca2+ influx exposed to the lowest evaluated concentration (0.00032%). When applied to the cosmetic baby formulation, although the presented model exhibited higher sensitivity by classifying as stinging formulations that had previously undergone clinical testing and were deemed non-stinging, the assay could serve as a valuable in vitro tool for predicting human eye stinging sensation and can be used as a tier 1 in an integrated testing strategy.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Cálcio / Cosméticos / Canais de Cátion TRPV Limite: Humans Idioma: En Revista: Toxicol In Vitro Assunto da revista: TOXICOLOGIA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Cálcio / Cosméticos / Canais de Cátion TRPV Limite: Humans Idioma: En Revista: Toxicol In Vitro Assunto da revista: TOXICOLOGIA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido