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Nitric oxide-releasing photocrosslinked chitosan cryogels.
Vieira de Almeida, Herllan; Escobar da Silva, Laura Caetano; Ganzarolli de Oliveira, Marcelo.
Afiliação
  • Vieira de Almeida H; Institute of Chemistry, University of Campinas, UNICAMP, Campinas, SP, Brazil.
  • Escobar da Silva LC; Institute of Chemistry, University of Campinas, UNICAMP, Campinas, SP, Brazil.
  • Ganzarolli de Oliveira M; Institute of Chemistry, University of Campinas, UNICAMP, Campinas, SP, Brazil. Electronic address: mgo@unicamp.br.
Nitric Oxide ; 146: 48-57, 2024 May 01.
Article em En | MEDLINE | ID: mdl-38579898
ABSTRACT
The highly porous morphology of chitosan cryogels, with submicrometric-sized pore cell walls, provides a large surface area which leads to fast water absorption and elevated swelling degrees. These characteristics are crucial for the applications of nitric oxide (NO) releasing biomaterials, in which the release of NO is triggered by the hydration of the material. In the present study, we report the development of chitosan cryogels (CS) with a porous structure of interconnected cells, with wall thicknesses in the range of 340-881 nm, capable of releasing NO triggered by the rapid hydration process. This property was obtained using an innovative strategy based on the functionalization of CS with two previously synthesized S-nitrosothiols S-nitrosothioglycolic acid (TGA(SNO)) and S-nitrosomercaptosuccinic acid (MSA(SNO)). For this purpose, CS was previously methacrylated with glycidyl methacrylate and subsequently submitted to photocrosslinking and freeze-drying processes. The photocrosslinked hydrogels thus obtained were then functionalized with TGA(SNO) and MSA(SNO) in reactions mediated by carbodiimide. After functionalization, the hydrogels were frozen and freeze-dried to obtain porous S-nitrosated chitosan cryogels with high swelling capacities. Through chemiluminescence measurements, we demonstrated that CS-TGA(SNO) and CS-MSA(SNO) cryogels spontaneously release NO upon water absorption at rates of 3.34 × 10-2 nmol mg-1 min-1 and 1.27 × 10-1 nmol mg-1 min-1, respectively, opening new perspectives for the use of CS as a platform for localized NO delivery in biomedical applications.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Quitosana / Criogéis / Óxido Nítrico Idioma: En Revista: Nitric Oxide Assunto da revista: BIOQUIMICA / QUIMICA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Quitosana / Criogéis / Óxido Nítrico Idioma: En Revista: Nitric Oxide Assunto da revista: BIOQUIMICA / QUIMICA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos