Detection of Escherichia coli O157H7 and Campylobacter jejuni in Bovine Carcasses in Two Slaughterhouses in Bio-Bío District, Chile.
Foodborne Pathog Dis
; 21(7): 409-415, 2024 Jul.
Article
em En
| MEDLINE
| ID: mdl-38568114
ABSTRACT
Escherichia coli O157H7 (E. coli O157H7) and Campylobacter jejuni (C. jejuni) are pathogenic microorganisms that can cause severe clinical symptoms in humans and are associated with bovine meat consumption. Specific monitoring for E. coli O157 H7 or C. jejuni in meat is not mandatory under Chilean regulations. In this study, we analyzed 544 samples for the detection of both microorganisms, obtained from 272 bovine carcasses (280 kg average) at two slaughterhouses in the Bio-Bío District, Chile. Sampling was carried out at post-shower of carcasses and after channel passage through the cold chamber. Eleven samples were found to be positive for E. coli O157H7 (4.0%) using microbiological and biochemical detection techniques and were subjected to a multiplex PCR to detect fliC and rfbE genes. Six samples (2.2%) were also found to be positive for the pathogenicity genes stx1, stx2, and eaeA. Twenty-two carcasses (8.0%) were found to be positive for C. jejuni using microbiological and biochemical detection techniques, but no sample with amplified mapA gene was found.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Campylobacter jejuni
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Matadouros
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Escherichia coli O157
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Proteínas de Escherichia coli
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Microbiologia de Alimentos
Limite:
Animals
País/Região como assunto:
America do sul
/
Chile
Idioma:
En
Revista:
Foodborne Pathog Dis
Assunto da revista:
CIENCIAS DA NUTRICAO
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MICROBIOLOGIA
/
PARASITOLOGIA
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
Chile
País de publicação:
Estados Unidos