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Biosurfactants production by marine yeasts isolated from zoanthids and characterization of an emulsifier produced by Yarrowia lipolytica LMS 24B.
Petra de Oliveira Barros, Vitória; Macedo Silva, Júlio Ricardo; Maciel Melo, Vânia Maria; Terceiro, Pâmela Santos; Nunes de Oliveira, Italo; Duarte de Freitas, Johnnatan; Francisco da Silva Moura, Orlando; Xavier de Araújo-Júnior, João; Erlanny da Silva Rodrigues, Erica; Maraschin, Marcelo; Thompson, Fabiano Lopes; Landell, Melissa Fontes.
Afiliação
  • Petra de Oliveira Barros V; Graduate Program in Genetics. Federal University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil; Institute of Biological and Health Sciences, Federal University of Alagoas, Maceió, AL, Brazil.
  • Macedo Silva JR; Institute of Biological and Health Sciences, Federal University of Alagoas, Maceió, AL, Brazil.
  • Maciel Melo VM; Department of Biology, Microbial Ecology and Biotechnology Laboratory (Lembiotech), Fortaleza, CE, Brazil.
  • Terceiro PS; Institute of Physics, Federal University of Alagoas, Maceió, AL, Brazil.
  • Nunes de Oliveira I; Institute of Physics, Federal University of Alagoas, Maceió, AL, Brazil.
  • Duarte de Freitas J; Federal Institute of Alagoas, Maceió, AL, Brazil.
  • Francisco da Silva Moura O; Federal Institute of Alagoas, Maceió, AL, Brazil.
  • Xavier de Araújo-Júnior J; Federal University of Alagoas, Pharmaceutical Sciences Institute, Maceió, AL, Brazil.
  • Erlanny da Silva Rodrigues E; Federal University of Alagoas, Pharmaceutical Sciences Institute, Maceió, AL, Brazil.
  • Maraschin M; Plant Morphogenesis and Biochemistry Laboratory, Federal University of Santa Catarina, Florianópolis, SC, Brazil.
  • Thompson FL; Institute of Biology, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
  • Landell MF; Institute of Biological and Health Sciences, Federal University of Alagoas, Maceió, AL, Brazil. Electronic address: melissa.landell@icbs.ufal.br.
Chemosphere ; 355: 141807, 2024 May.
Article em En | MEDLINE | ID: mdl-38552803
ABSTRACT
The present study investigates the potential for biosurfactant production of 19 marine yeast species obtained from zoanthids. Using the emulsification index test to screen the samples produced by the marine yeasts, we verified that five isolates exhibited an emulsification index ≥50%. Additional tests were performed on such isolates, including oil displacement, drop collapse, Parafilm M assay, and surface tension measurement. The tolerance of produced biosurfactants for environmental conditions was also analyzed, especially considering the media's temperature, pH, and salinity. Moreover, the surfactant's ability to emulsify different hydrocarbon sources and to metabolize kerosene as the sole carbon source was evaluated in vitro. Our results demonstrate that yeast biosurfactants can emulsify hydrocarbon sources under different physicochemical conditions and metabolize kerosene as a carbon source. Considering the Yarrowia lipolytica LMS 24B as the yeast model for biosurfactant production from the cell's wall biomass, emulsification indexes of 61.2% were obtained, even at a high temperature of 120 °C. Furthermore, the Fourier-transform middle infrared spectroscopy (FTIR) analysis of the biosurfactant's chemical composition revealed the presence of distinct functional groups assigned to a glycoprotein complex. Considering the status of developing new bioproducts and bioprocesses nowadays, our findings bring a new perspective to biosurfactant production by marine yeasts, especially Y. lipolytica LMS 24B. In particular, the presented results validate the relevance of marine environments as valuable sources of genetic resources, i.e., yeast strains capable of metabolizing and emulsifying petroleum derivatives.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Petróleo / Yarrowia Idioma: En Revista: Chemosphere Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Petróleo / Yarrowia Idioma: En Revista: Chemosphere Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido