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New artificial hematophagy system with attractive polymeric biofilm for maintenance of Culex quinquefasciatus (Diptera: Culicidae) in the laboratory.
Milech, Angelita; Braga, Caroline Quintana; Dos Santos Bermann, Carolina; de Souza, Jaqueline Ferreira; Fajardo, André Ricardo; Vianna, Élvia Silveira; Oliveira, Camila Belmonte.
Afiliação
  • Milech A; Department of Microbiology and Parasitology, Institute of Biology, Federal University of Pelotas (UFPel), Pelotas, RS, Brazil.
  • Braga CQ; Department of Microbiology and Parasitology, Institute of Biology, Federal University of Pelotas (UFPel), Pelotas, RS, Brazil.
  • Dos Santos Bermann C; Department of Microbiology and Parasitology, Institute of Biology, Federal University of Pelotas (UFPel), Pelotas, RS, Brazil.
  • de Souza JF; Center for Sciences Chemical, Pharmaceutical and Food Sciences, Federal University of Pelotas (UFPel), Pelotas, RS, Brazil.
  • Fajardo AR; Center for Sciences Chemical, Pharmaceutical and Food Sciences, Federal University of Pelotas (UFPel), Pelotas, RS, Brazil.
  • Vianna ÉS; Department of Microbiology and Parasitology, Institute of Biology, Federal University of Pelotas (UFPel), Pelotas, RS, Brazil.
  • Oliveira CB; Department of Microbiology and Parasitology, Institute of Biology, Federal University of Pelotas (UFPel), Pelotas, RS, Brazil. camilabelmontevet@yahoo.com.br.
Parasit Vectors ; 17(1): 136, 2024 Mar 15.
Article em En | MEDLINE | ID: mdl-38491527
ABSTRACT

BACKGROUND:

Maintaining mosquito colonies in the laboratory requires a blood supply so that females' oocytes can mature and oviposition can take place. In this study, a new artificial hematophagy system for colonization and maintenance of Culex quinquefasciatus in the laboratory was developed and tested.

METHODS:

We developed an attractive polymeric biofilm including 25% L-lactic acid for use as a membrane in an artificial hematophagy system and compared the feeding rate of females with Parafilm-M®. We also evaluated the oviposition rate, larval survival and adult emergence of females fed through the attractive biofilm.

RESULTS:

The average percentage of female Cx. quinquefasciatus fed through the attractive biofilm was 87%, while only 20% became engorged with Parafilm-M® (p < 0.0001). Feeding through the attractive biofilm developed in this study produced high levels of evaluated biological parameters; the percentage of egg laying by females that underwent artificial hematophagy through the biofilm was 90%, with an average of 158 eggs per raft. From these eggs, 97% of the larvae hatched, of which 95% reached the pupal stage. The adult emergence rate corresponded to 93% of pupae.

CONCLUSIONS:

Insects fed with attractant through the biofilm system had a higher engorgement rate compared to those fed through Parafilm-M®. Our study is preliminary and suggests that polymeric biofilm has great potential for artificially feeding mosquitoes in the laboratory. Based on this research, new studies will be carried out with biofilm and different systems.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Culex / Culicidae Limite: Animals Idioma: En Revista: Parasit Vectors Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Culex / Culicidae Limite: Animals Idioma: En Revista: Parasit Vectors Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido