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Effect of High Sodium Intake on Gut Tight Junctions' Structure and Permeability to Bacterial Toxins in a Rat Model of Chronic Kidney Disease.
Villela-Torres, María de la Luz; Prado-Uribe, María-Del-Carmen; Díaz, Marcela Ávila; Pablo, Héctor Quezada; Soria-Castro, Elizabeth; Escofet, Nuria Esturau; Maldonado, Catalina Elizabeth Flores; Paniagua, Ramón.
Afiliação
  • Villela-Torres ML; Medical Research Unit in Nephrological Diseases, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Mexico City, Mexico.
  • Prado-Uribe MD; Medical Research Unit in Nephrological Diseases, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Mexico City, Mexico.
  • Díaz MÁ; Medical Research Unit in Nephrological Diseases, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Mexico City, Mexico.
  • Pablo HQ; Immunnology and Proteomics Research Lab, Hospital Infantil de México Federico Gómez, Mexico City, Mexico.
  • Soria-Castro E; Cardiovascular Biomedicine Department, Instituto Nacional de Cardiología Ignacio Chávez, Mexico City, Mexico.
  • Escofet NE; Physical Chemistry Department, Laboratorio Universitario de Resonancia Magnética Nuclear, Instituto de Química, Universidad Nacional Autónoma de México, Mexico City, Mexico.
  • Maldonado CEF; Physiology, Biophysics and Neurosciences Department from Cinvestav Unidad Zacatenco, Instituto Politécnico Nacional, Mexico City, Mexico.
  • Paniagua R; Medical Research Unit in Nephrological Diseases, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Mexico City, Mexico. Electronic address: jose.paniagua@imss.gob.mx.
Arch Med Res ; 55(3): 102969, 2024 Apr.
Article em En | MEDLINE | ID: mdl-38484487
ABSTRACT

INTRODUCTION:

Uremic toxicity changes the gut structure and permeability, allowing bacterial toxins to translocate from the lumen to the blood during chronic kidney failure (CKD). Clinical fluid overload and tissue edema without uremia have similar effects but have not been adequately demonstrated and analyzed in CKD.

AIMS:

To investigate the effect of sodium intake on the plasma concentration of gut-derived uremic toxins, indoxyl sulfate (IS), and p-cresyl sulfate (pCS) and the expression of genes and proteins of epithelial gut tight junctions in a rat model of CKD.

METHODS:

Sham-operated (control group, CG) and five-sixths nephrectomized (5/6Nx) Sprague-Dawley rats were randomly assigned to low (LNa), normal (NNa), or high sodium (HNa) diets., Animals were then sacrificed at 8 and 12 weeks and analyzed for IS and pCS plasma concentrations, as well as for gene and protein expression of thigh junction proteins, and transmission electron microscopy (TEM) in colon fragments.

RESULTS:

The HNa 5/6Nx groups had higher concentrations of IS and pCS than CG, NNa, and LNa at eight and twelve weeks. Furthermore, HNa 5/6Nx groups had reduced expression of the claudin-4 gene and protein than CG, NNa, and LNa. HNa had reduced occludin gene expression compared to CG. Occludin protein expression was more reduced in HNa than in CG, NNa, and LNa. The gut epithelial tight junctions appear dilated in HNa compared to NNa and LNa in TEM.

CONCLUSION:

Dietary sodium intake and fluid overload have a significant role in gut epithelial permeability in the CKD model.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Toxinas Bacterianas / Sódio na Dieta / Insuficiência Renal Crônica Limite: Animals Idioma: En Revista: Arch Med Res Assunto da revista: MEDICINA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: México País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Toxinas Bacterianas / Sódio na Dieta / Insuficiência Renal Crônica Limite: Animals Idioma: En Revista: Arch Med Res Assunto da revista: MEDICINA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: México País de publicação: Estados Unidos