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Liposomal nanoformulations with trans-caryophyllene and caryophyllene oxide: do they have an inhibitory action on the efflux pumps NorA, Tet(K), MsrA, and MepA?
Santana, Jorge Ederson Gonçalves; Oliveira-Tintino, Cícera Datiane de Morais; Alencar, Gabriel Gonçalves; Siqueira, Gustavo Miguel; Almeida-Bezerra, José Weverton; Viana Rodrigues, João Pedro; Pinheiro Gonçalves, Vanessa Barbosa; Nicolete, Roberto; Tintino, Saulo Relison; Coutinho, Henrique Douglas Melo; Silva, Teresinha Gonçalves da.
Afiliação
  • Santana JEG; Departamento de Antibióticos, Universidade Federal de Pernambuco (UFPE), Recife, 50670 901, Pernambuco, Brazil.
  • Oliveira-Tintino CDM; Departament of Biological Chemistry, Universidade Regional Do Cariri (URCA), Crato, 63105-010, Ceará, Brazil. Electronic address: datianemorais@hotmail.com.
  • Alencar GG; Departament of Biological Chemistry, Universidade Regional Do Cariri (URCA), Crato, 63105-010, Ceará, Brazil.
  • Siqueira GM; Departament of Biological Chemistry, Universidade Regional Do Cariri (URCA), Crato, 63105-010, Ceará, Brazil.
  • Almeida-Bezerra JW; Departament of Biological Chemistry, Universidade Regional Do Cariri (URCA), Crato, 63105-010, Ceará, Brazil.
  • Viana Rodrigues JP; Oswaldo Cruz Foundation (Fiocruz Ceará), Eusebio, 61773-270, Ceará, Brazil.
  • Pinheiro Gonçalves VB; Oswaldo Cruz Foundation (Fiocruz Ceará), Eusebio, 61773-270, Ceará, Brazil.
  • Nicolete R; Oswaldo Cruz Foundation (Fiocruz Ceará), Eusebio, 61773-270, Ceará, Brazil.
  • Tintino SR; Departament of Biological Chemistry, Universidade Regional Do Cariri (URCA), Crato, 63105-010, Ceará, Brazil.
  • Coutinho HDM; Departament of Biological Chemistry, Universidade Regional Do Cariri (URCA), Crato, 63105-010, Ceará, Brazil.
  • Silva TGD; Departamento de Antibióticos, Universidade Federal de Pernambuco (UFPE), Recife, 50670 901, Pernambuco, Brazil.
Chem Biol Interact ; 393: 110945, 2024 Apr 25.
Article em En | MEDLINE | ID: mdl-38460934
ABSTRACT
This study aimed to evaluate the antibacterial and inhibitory action of NorA, Tet(K), MsrA and MepA efflux pumps in S. aureus strains using the sesquiterpenes named trans-caryophyllene and caryophyllene oxide, both isolated and encapsulated in liposomes. The antibacterial and inhibitory action of these efflux pumps was evaluated through the serial microdilution test in 96-well microplates. Each sesquiterpene and liposome/sesquiterpene was combined with antibiotics and ethidium bromide (EtBr). The antibiotics named norfloxacin, tetracycline and erythromycin were used. The 1199 B, IS-58, RN4220 and K2068 S. aureus strains carrying NorA, Tet(K), MsrA and MepA, respectively, were tested. In the fluorescence measurement test, K2068 S. aureus was incubated with the sesquiterpenes and EtBr, and the fluorescence emission by EtBr was measured. The tested substances did not show direct antibacterial activity, with MIC >1024 µg/mL. Nonetheless, the isolated trans-caryophyllene and caryophyllene oxide reduced the MIC of antibiotics and EtBr, indicating inhibition of NorA, Tet(K) and MsrA. In the fluorescence test, these same sesquiterpenes increased fluorescence emission, indicating inhibition of MepA. Therefore, the sesquiterpenes named trans-caryophyllene and caryophyllene oxide did not show direct antibacterial action; however, in their isolated form, they showed possible inhibitory action on NorA, Tet(K), MsrA and MepA efflux pumps. They may also act in antibiotic potentiation. Further studies are needed to identify the mechanisms involved in antibiotic potentiation and efflux pump inhibitory action.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Staphylococcus aureus / Lipossomos Idioma: En Revista: Chem Biol Interact Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Irlanda
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Staphylococcus aureus / Lipossomos Idioma: En Revista: Chem Biol Interact Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Irlanda