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Comparison of DNA damage in granulosa cells of women undergoing controlled ovarian stimulation in in vitro fertilization protocols with the recombinant human follicle-stimulating hormones Corneumon®, Gonal-F®, Pergoveris® and Puregon®: a randomized trial.
Alcalá-Sánchez, Ximena; Cuapio-Padilla, Pedro; Salazar-López, Carlos; Rodríguez, Ricardo; Teteltitla, Mario; Bahena, Iván; Betancourt, Miguel; Casas, Eduardo; Casillas, Fahiel; López, Alma; Bonilla, Edmundo.
Afiliação
  • Alcalá-Sánchez X; Department of Health Sciences, Metropolitan Autonomous University-Iztapalapa Campus, 09340, Mexico City, Mexico.
  • Cuapio-Padilla P; Master's Degree Program in Animal Reproduction Biology, Metropolitan Autonomous University-Iztapalapa Campus, 09340, Mexico City, Mexico.
  • Salazar-López C; HISPAREP Assisted Reproduction Clinic, Hospital Español, 11520 CDMX, Mexico City, Mexico.
  • Rodríguez R; HISPAREP Assisted Reproduction Clinic, Hospital Español, 11520 CDMX, Mexico City, Mexico.
  • Teteltitla M; HISPAREP Assisted Reproduction Clinic, Hospital Español, 11520 CDMX, Mexico City, Mexico.
  • Bahena I; Department of Health Sciences, Metropolitan Autonomous University-Iztapalapa Campus, 09340, Mexico City, Mexico.
  • Betancourt M; Department of Health Sciences, Metropolitan Autonomous University-Iztapalapa Campus, 09340, Mexico City, Mexico.
  • Casas E; Department of Health Sciences, Metropolitan Autonomous University-Iztapalapa Campus, 09340, Mexico City, Mexico.
  • Casillas F; Department of Health Sciences, Metropolitan Autonomous University-Iztapalapa Campus, 09340, Mexico City, Mexico.
  • López A; Department of Biology of Reproduction, Metropolitan Autonomous University-Iztapalapa Campus, 09340, Mexico City, Mexico.
  • Bonilla E; Department of Health Sciences, Metropolitan Autonomous University-Iztapalapa Campus, 09340, Mexico City, Mexico.
Arch Gynecol Obstet ; 309(5): 2107-2114, 2024 05.
Article em En | MEDLINE | ID: mdl-38441601
ABSTRACT

PURPOSE:

To compare the DNA damage in granulosa cells (GCs) of women undergoing ovarian-stimulated cycles with four widely used recombinant human follicle-stimulating hormones (rhFSH) in in vitro fertilization (IVF) protocols (Corneumon®, Gonal-F®, Pergoveris® and Puregon®).

METHODS:

A randomized trial was carried out at a Mexican hospital. GCs were isolated from 18 women with infertility undergoing assisted reproductive techniques (ART). Four controlled ovarian stimulation (COS) protocols including Corneumon®, Gonal-F®, Pergoveris® or Puregon® were used. GCs DNA damage was assessed by the Comet assay. Two parameters were measured comet tail length (CTL), and Olive tail moment (OTM, the percentage of DNA in the tail multiplied by the distance between the center of the tail and head).

RESULTS:

Use of the different hrFSH in COS caused variable and statistically significant levels of DNA damage in GCs of infertile women. CTL was similar in the Corneumon® and Pergoveris® groups (mean values of 48.73 and 55.18, respectively) and Corneumon® CTL was significantly lower compared to the Gonal-F® and Puregon® groups (mean values of 61.98 and 91.17, respectively). Mean OTM values were significantly lower in Corneumon® and Pergoveris® groups, compared to Gonal-F® and Puregon® groups (25.59, 27.35, 34.76, and 47.27, respectively).

CONCLUSION:

Use of Corneumon® and Pergoveris® in COS caused statistically significantly lower levels of DNA damage in GCs of infertile women undergoing ART, which could potentially correlate with better reproductive outcomes.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Hormônio Luteinizante / Infertilidade Feminina Limite: Female / Humans Idioma: En Revista: Arch Gynecol Obstet Assunto da revista: GINECOLOGIA / OBSTETRICIA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: México País de publicação: Alemanha
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Hormônio Luteinizante / Infertilidade Feminina Limite: Female / Humans Idioma: En Revista: Arch Gynecol Obstet Assunto da revista: GINECOLOGIA / OBSTETRICIA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: México País de publicação: Alemanha