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Serum response factor promoting axonal regeneration by activating the Ras-Raf-Cofilin signaling pathway after the spinal cord injury.
Gao, Limin; Zhang, Chen; Zhu, Yonglin; Zhang, Naili; Zhang, Chunlei; Zhou, Shuai; Feng, Guoying; Huang, Fei; Zhang, Luping.
Afiliação
  • Gao L; Institute of Neurobiology, Binzhou Medical University, Yantai, Shandong Province, China.
  • Zhang C; Department of Neurobiology, School of Basic Medical Sciences, Capital Medical University, Beijing, China.
  • Zhu Y; Institute of Neurobiology, Binzhou Medical University, Yantai, Shandong Province, China.
  • Zhang N; Experimental Neurosurgery, Department of Neurosurgery, Neuroscience Center, Frankfurt University Hospital, Frankfurt am Main, Germany.
  • Zhang C; Department of Bone and Joint, Yantai Affiliated Hospital of Binzhou Medical University, Yantai, Shandong, China.
  • Zhou S; Institute of Neurobiology, Binzhou Medical University, Yantai, Shandong Province, China.
  • Feng G; Institute of Neurobiology, Binzhou Medical University, Yantai, Shandong Province, China.
  • Huang F; Institute of Neurobiology, Binzhou Medical University, Yantai, Shandong Province, China.
  • Zhang L; Institute of Neurobiology, Binzhou Medical University, Yantai, Shandong Province, China.
CNS Neurosci Ther ; 30(2): e14585, 2024 02.
Article em En | MEDLINE | ID: mdl-38421133
ABSTRACT

INTRODUCTION:

Serum response factor (SRF) is important in muscle development, tissue repair, and neuronal regulation.

OBJECTIVES:

This research aims to thoroughly examine the effects of SRF on spinal cord injury (SCI) and its ability to significantly impact the recovery and regeneration of neuronal axons.

METHODS:

The researchers created rat models of SCI and scratch injury to primary spinal cord neurons to observe the expression of relevant factors after neuronal injury.

RESULTS:

We found that the SRF, Ras, Raf, and cofilin levels increased after injury and gradually returned to normal levels. Afterward, researchers gave rats with SCI an SRF inhibitor (CCG1423) and studied the effects with nuclear magnetic resonance and transmission electron microscopy. The SRF inhibitor rodents had worse spinal cord recovery and axon regrowth than the control group. And the apoptosis of primary neurons after scratch injury was significantly higher in the SRF inhibitor group. Additionally, the researchers utilized lentiviral transfection to modify the SRF expression in neurons. SRF overexpression increased neuron migration while silencing SRF decreased it. Finally, Western blotting and RT-PCR were conducted to examine the expression changes of related factors upon altering SRF expression. The results revealed SRF overexpression increased Ras, Raf, and cofilin expression. Silencing SRF decreased Ras, Raf, and Cofilin expression.

CONCLUSION:

Based on our research, the SRF promotes axonal regeneration by activating the "Ras-Raf-Cofilin" signaling pathway.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Traumatismos da Medula Espinal / Fatores de Despolimerização de Actina Limite: Animals Idioma: En Revista: CNS Neurosci Ther Assunto da revista: NEUROLOGIA / TERAPEUTICA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Traumatismos da Medula Espinal / Fatores de Despolimerização de Actina Limite: Animals Idioma: En Revista: CNS Neurosci Ther Assunto da revista: NEUROLOGIA / TERAPEUTICA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China País de publicação: Reino Unido