Renal proximal tubule-on-a-chip in PDMS: fabrication, functionalization, and RPTEC:HUVEC co-culture evaluation.

Guimaraes, Ana Paula Pereira; Calori, Italo Rodrigo; Stilhano, Roberta Sessa; Tedesco, Antonio Claudio

Guimaraes, Ana Paula Pereira; Calori, Italo Rodrigo; Stilhano, Roberta Sessa; Tedesco, Antonio Claudio.

Afiliação

Guimaraes APP; Department of Chemistry, Center of Nanotechnology and Tissue Engineering- Photobiology and Photomedicine Research Group, Faculty of Philosophy, Sciences and Letters of Ribeirão Preto, University of São Paulo, São Paulo, Ribeirão Preto 14040-901, Brazil.
Calori IR; Department of Chemistry, Center of Nanotechnology and Tissue Engineering- Photobiology and Photomedicine Research Group, Faculty of Philosophy, Sciences and Letters of Ribeirão Preto, University of São Paulo, São Paulo, Ribeirão Preto 14040-901, Brazil.
Stilhano RS; Pharmaceutical Engineering and 3D Printing (PharmE3D) Labs, Department of Pharmaceutics and Drug Delivery, School of Pharmacy, The University of Mississippi, University, Oxford, MS 38677, United States of America.
Tedesco AC; Department of Physiological Sciences, Santa Casa de Sao Paulo School of Medical Sciences, Sao Paulo, Brazil.

Biofabrication ; 16(2)2024 Mar 07.

Article em En | MEDLINE | ID: mdl-38408383

ABSTRACT

ABSTRACT

'On-a-chip' technology advances the development of physiologically relevant organ-mimicking architecture by integrating human cells into three-dimensional microfluidic devices. This method also establishes discrete functional units, faciliting focused research on specific organ components. In this study, we detail the development and assessment of a convoluted renal proximal tubule-on-a-chip (PT-on-a-chip). This platform involves co-culturing Renal Proximal Tubule Epithelial Cells (RPTEC) and Human Umbilical Vein Endothelial Cells (HUVEC) within a polydimethylsiloxane microfluidic device, crafted through a combination of 3D printing and molding techniques. Our PT-on-a-chip significantly reduced high glucose level, exhibited albumin uptake, and simulated tubulopathy induced by amphotericin B. Remarkably, the RPTECHUVEC co-culture exhibited efficient cell adhesion within 30 min on microchannels functionalized with plasma, 3-aminopropyltriethoxysilane, and type-I collagen. This approach significantly reduced the required incubation time for medium perfusion. In comparison, alternative methods such as plasma and plasma plus polyvinyl alcohol were only effective in promoting cell attachment to flat surfaces. The PT-on-a-chip holds great promise as a valuable tool for assessing the nephrotoxic potential of new drug candidates, enhancing our understanding of drug interactions with co-cultured renal cells, and reducing the need for animal experimentation, promoting the safe and ethical development of new pharmaceuticals.

Assuntos

Células Epiteliais; Túbulos Renais Proximais; Animais; Humanos; Células Endoteliais da Veia Umbilical Humana; Técnicas de Cocultura; Túbulos Renais Proximais/metabolismo; Dispositivos Lab-On-A-Chip

Palavras-chave

3D printing; PDMS; kidney; organ-on-chip; renal tubule

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Células Epiteliais / Túbulos Renais Proximais Limite: Animals / Humans Idioma: En Revista: Biofabrication Assunto da revista: BIOTECNOLOGIA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido

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