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Biofilm formation by Staphylococcus pseudintermedius on titanium implants.
Pesset, Camilla Malcher; Fonseca, Carolina O da; Antunes, Milena; Santos, Ana Luiza L Dos; Teixeira, Izabel Melo; Ferreira, Eliane de Oliveira; Penna, Bruno.
Afiliação
  • Pesset CM; Laboratory of Gram-Positive Cocci, Biomedical Institute, Federal Fluminense University, Niterói, Brazil.
  • Fonseca COD; Laboratory of Gram-Positive Cocci, Biomedical Institute, Federal Fluminense University, Niterói, Brazil.
  • Antunes M; Laboratory of Gram-Positive Cocci, Biomedical Institute, Federal Fluminense University, Niterói, Brazil.
  • Santos ALLD; Laboratory of Gram-Positive Cocci, Biomedical Institute, Federal Fluminense University, Niterói, Brazil.
  • Teixeira IM; Laboratory of Gram-Positive Cocci, Biomedical Institute, Federal Fluminense University, Niterói, Brazil.
  • Ferreira EO; Institute of Microbiology Paulo de Góes, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
  • Penna B; Laboratory of Gram-Positive Cocci, Biomedical Institute, Federal Fluminense University, Niterói, Brazil.
Biofouling ; 40(1): 88-97, 2024 Jan.
Article em En | MEDLINE | ID: mdl-38407199
ABSTRACT
Osteomyelitis often involves Staphylococcus spp. as the isolated genus in domestic animal cases. Implant-related infections, frequently associated with biofilm-forming microorganisms like staphylococci species, necessitate careful material selection. This study assessed biofilm formation by Staphylococcus pseudintermedius on titanium nuts used in veterinary orthopaedic surgery. Biofilm quantification employed safranin staining and spectrophotometric measurement, while bacterial counts were determined in colony-forming units (CFU). Scanning Electron Microscopy (SEM) evaluated the biofilm morphology on the surface of titanium nuts. All samples had CFU counts. Absorbance values that evidence biofilm formation were observed in seven of the eight samples tested. SEM images revealed robust bacterial colonization, and significant extracellular polymeric substance production, and the negative control displayed surface irregularities on the nut. Whole genome sequencing revealed accessory Gene Regulator (agr) type III in six samples, agr IV and agr II in two each. Genes encoding hlb, luk-S, luk-F, siet, se_int, and the icaADCB operon were identified in all sequenced samples. Other exfoliative toxins were absent. Biofilm formation by S. pseudintermedius was detected in all samples, indicating the susceptibility of orthopaedic titanium alloys to adhesion and biofilm formation by veterinary species. The biofilm formation capacity raises concerns about potential post-surgical complications and associated costs.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Infecções Estafilocócicas / Biofilmes Limite: Animals Idioma: En Revista: Biofouling Assunto da revista: BIOLOGIA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Infecções Estafilocócicas / Biofilmes Limite: Animals Idioma: En Revista: Biofouling Assunto da revista: BIOLOGIA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido