Your browser doesn't support javascript.
loading
The Effects In Vitro of Photobiomodulation Over Fibroblasts and Extracellular Matrix.
Cavalcanti, Marcos Fernando Xisto Braga; Cabette, Roseli Alves; Moraes, Angelina Cirelli; Diomede, Francesca; Trubiani, Oriana; Maria, Durvanei Augusto.
Afiliação
  • Cavalcanti MFXB; Laboratory of Development and Innovation of Instituto Butantan, São Paulo, Brazil.
  • Cabette RA; Dental Clinic of the child and the adult of Nove de Julho University, São Paulo, Brazil.
  • Moraes AC; Laboratory of Development and Innovation of Instituto Butantan, São Paulo, Brazil.
  • Diomede F; Laboratory of Development and Innovation of Instituto Butantan, São Paulo, Brazil.
  • Trubiani O; Department of Medical, Oral and Biotechnological Sciences, Università degli Studi G. d'Annunzio, Pescara-Chieti, Italy.
  • Maria DA; Department of Medical, Oral and Biotechnological Sciences, Università degli Studi G. d'Annunzio, Pescara-Chieti, Italy.
Photobiomodul Photomed Laser Surg ; 42(2): 140-147, 2024 Feb.
Article em En | MEDLINE | ID: mdl-38393824
ABSTRACT

Objective:

The objective of this study is to evaluate the potential effects of photobiomodulation (PBM) on cell proliferation and extracellular matrix production of human fibroblasts (FN1) cultured in 2D.

Background:

Patients with healing difficulties suffer injuries that take time to recover. In addition, aging can be seen in our faces daily when we look in the mirror; in both situations, collagen production is reduced. Fibroblasts act in the beginning and at the end of the inflammation phase, signaling to immune agents, and platelets, and producing collagen, coordinating repair. PBM increases cell viability, proliferation, and mRNA production.

Methods:

Human fibroblasts were irradiated three times after cell seed (after 24, 48, and 72 h) using a gallium-aluminum arsenideGaAlAs low-level laser (LLL). Cell viability, proliferative response, synthesis of collagen types I and III, and soluble collagen production were analyzed. The statistical significance of differences between groups was determined using unpaired one-way analysis of variance (ANOVA) p < 0.05.

Results:

PBM increased significantly the number of fibroblasts, and the production of collagen types I (Col I) and III (Col III), after three sessions of LLL with 2.5 J per session, every 24 h, for 3 consecutive days; total energy delivered after 72 h is 7.5 J.

Conclusions:

This energy density of LLL increases fibroblast proliferation and collagen production in vitro without side effects.
Assuntos
Palavras-chave
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Terapia com Luz de Baixa Intensidade Limite: Humans Idioma: En Revista: Photobiomodul Photomed Laser Surg Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Terapia com Luz de Baixa Intensidade Limite: Humans Idioma: En Revista: Photobiomodul Photomed Laser Surg Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos