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Bimodal distribution of azole susceptibility in Sporothrix brasiliensis isolates in Brazil.
Ribeiro Dos Santos, Amanda; Gade, Lalitha; Misas, Elizabeth; Litvintseva, Anastasia P; Nunnally, Natalie S; Parnell, Lindsay A; Rajeev, Malavika; de Souza Carvalho Melhem, Marcia; Takahashi, Juliana Possato Fernandes; Oliboni, Gabriel Manzi; Bonfieti, Lucas Xavier; Araujo, Lisandra Siufi; Cappellano, Paola; Venturini, James; Lockhart, Shawn R; Sexton, D Joseph.
Afiliação
  • Ribeiro Dos Santos A; Oak Ridge Institute for Science and Education, Oak Ridge, Tennessee, USA.
  • Gade L; Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
  • Misas E; Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
  • Litvintseva AP; Oak Ridge Institute for Science and Education, Oak Ridge, Tennessee, USA.
  • Nunnally NS; Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
  • Parnell LA; Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
  • Rajeev M; Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
  • de Souza Carvalho Melhem M; Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
  • Takahashi JPF; Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
  • Oliboni GM; School of Medicine, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brazil.
  • Bonfieti LX; Graduate Program in Tropical Diseases, Universidade Estadual Paulista, Botucatu, SP, Brazil.
  • Araujo LS; Parasitology and Mycology Center, Instituto Adolfo Lutz, São Paulo, SP, Brazil.
  • Cappellano P; School of Medicine, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brazil.
  • Venturini J; Parasitology and Mycology Center, Instituto Adolfo Lutz, São Paulo, SP, Brazil.
  • Lockhart SR; Graduate Program in Sciences, Coordenadoria de Controle de Doenças, Secretary of Health, São Paulo, Brazil.
  • Sexton DJ; Parasitology and Mycology Center, Instituto Adolfo Lutz, São Paulo, SP, Brazil.
Antimicrob Agents Chemother ; 68(4): e0162023, 2024 Apr 03.
Article em En | MEDLINE | ID: mdl-38385701
ABSTRACT
Sporothrix brasiliensis is an emerging zoonotic fungal pathogen that can be difficult to treat. Antifungal susceptibility testing was performed on the mold phase of a convenience sample of 61 Sporothrix spp. isolates from human and cat sporotrichosis cases in Brazil using the Clinical and Laboratory Standards Institute standard M38. A bimodal distribution of azole susceptibility was observed with 50% (28/56) of S. brasiliensis isolates showing elevated itraconazole minimum inhibitory concentrations ≥16 µg/mL. Phylogenetic analysis found the in vitro resistant isolates were not clonal and were distributed across three different S. brasiliensis clades. Single nucleotide polymorphism (SNP) analysis was performed to identify potential mechanisms of in vitro resistance. Two of the 28 resistant isolates (MIC ≥16 mg/L) had a polymorphism in the cytochrome P450 gene, cyp51, corresponding to the well-known G448S substitution inducing azole resistance in Aspergillus fumigatus. SNPs corresponding to other known mechanisms of azole resistance were not identified in the remaining 26 in vitro resistant isolates.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Esporotricose / Sporothrix Limite: Humans País/Região como assunto: America do sul / Brasil Idioma: En Revista: Antimicrob Agents Chemother Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Estados Unidos País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Esporotricose / Sporothrix Limite: Humans País/Região como assunto: America do sul / Brasil Idioma: En Revista: Antimicrob Agents Chemother Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Estados Unidos País de publicação: Estados Unidos