Your browser doesn't support javascript.
loading
Biodegradation of Polystyrene by Galleria mellonella: Identification of Potential Enzymes Involved in the Degradative Pathway.
Venegas, Sebastián; Alarcón, Carolina; Araya, Juan; Gatica, Marcell; Morin, Violeta; Tarifeño-Saldivia, Estefanía; Uribe, Elena.
Afiliação
  • Venegas S; Department of Biochemistry and Molecular Biology, Faculty of Biological Sciences, University of Concepción, Concepción 4070409, Chile.
  • Alarcón C; Department of Biochemistry and Molecular Biology, Faculty of Biological Sciences, University of Concepción, Concepción 4070409, Chile.
  • Araya J; Department of Instrumental Analysis, Faculty of Pharmacy, University of Concepción, Concepción 4070409, Chile.
  • Gatica M; Department of Biochemistry and Molecular Biology, Faculty of Biological Sciences, University of Concepción, Concepción 4070409, Chile.
  • Morin V; Department of Biochemistry and Molecular Biology, Faculty of Biological Sciences, University of Concepción, Concepción 4070409, Chile.
  • Tarifeño-Saldivia E; Department of Biochemistry and Molecular Biology, Faculty of Biological Sciences, University of Concepción, Concepción 4070409, Chile.
  • Uribe E; Department of Instrumental Analysis, Faculty of Pharmacy, University of Concepción, Concepción 4070409, Chile.
Int J Mol Sci ; 25(3)2024 Jan 27.
Article em En | MEDLINE | ID: mdl-38338857
ABSTRACT
Galleria mellonella is a lepidopteran whose larval stage has shown the ability to degrade polystyrene (PS), one of the most recalcitrant plastics to biodegradation. In the present study, we fed G. mellonella larvae with PS for 54 days and determined candidate enzymes for its degradation. We first confirmed the biodegradation of PS by Fourier transform infrared spectroscopy- Attenuated total reflectance (FTIR-ATR) and then identified candidate enzymes in the larval gut by proteomic analysis using liquid chromatography with tandem mass spectrometry (LC-MS/MS). Two of these proteins have structural similarities to the styrene-degrading enzymes described so far. In addition, potential hydrolases, isomerases, dehydrogenases, and oxidases were identified that show little similarity to the bacterial enzymes that degrade styrene. However, their response to a diet based solely on polystyrene makes them interesting candidates as a potential new group of polystyrene-metabolizing enzymes in eukaryotes.
Assuntos
Palavras-chave
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Poliestirenos / Mariposas Tipo de estudo: Diagnostic_studies Limite: Animals Idioma: En Revista: Int J Mol Sci Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Chile País de publicação: Suíça
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Poliestirenos / Mariposas Tipo de estudo: Diagnostic_studies Limite: Animals Idioma: En Revista: Int J Mol Sci Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Chile País de publicação: Suíça