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CFTR expression in human salivary gland acinar cells.
Muñoz, Manuel; Acevedo, Alejandro; Ovitt, Catherine E; Luitje, Martha E; Maruyama, Eri O; Catalán, Marcelo A.
Afiliação
  • Muñoz M; Instituto de Nutrición y Tecnología de los Alimentos, Universidad de Chile, Santiago, Chile.
  • Acevedo A; Instituto de Nutrición y Tecnología de los Alimentos, Universidad de Chile, Santiago, Chile.
  • Ovitt CE; Department of Biomedical Genetics, University of Rochester Medical Center, Rochester, New York, United States.
  • Luitje ME; Department of Otolaryngology, University of Rochester Medical Center, Rochester, New York, United States.
  • Maruyama EO; Department of Biomedical Genetics, University of Rochester Medical Center, Rochester, New York, United States.
  • Catalán MA; Instituto de Fisiología, Facultad de Medicina, Universidad Austral de Chile, Valdivia, Chile.
Am J Physiol Cell Physiol ; 326(3): C742-C748, 2024 03 01.
Article em En | MEDLINE | ID: mdl-38284125
ABSTRACT
The key role of CFTR in secretory epithelia has been extensively documented. Additionally, CFTR plays a significant role in ion absorption in exocrine glands, including salivary and sweat glands. Most of the knowledge about CFTR expression comes from animal models such as the mouse or the rat, but there is limited information about CFTR expression in human tissues. In the present study, we assessed the expression of CFTR in human submandibular and parotid glands. Consistent with findings in rodent salivary glands, our immunolocalization studies show that CFTR is expressed in duct cells. However, CFTR expression in human salivary glands differs from that in rodents, as immunolocalization and single-cell RNA sequencing analysis from a previous study performed in the human parotid gland revealed the presence of CFTR protein and transcripts within a distinct cell cluster. Based on cell marker expression, this cluster corresponds to acinar cells. To obtain functional evidence supporting CFTR expression, we isolated human parotid acinar cells through collagenase digestion. Acinar cells displayed an anion conductance that was activated in response to cAMP-increasing agents and was effectively blocked by CFTRInh172, a known CFTR blocker. This study provides novel evidence of CFTR expression within acinar cells of human salivary glands. This finding challenges the established model positioning CFTR exclusively in duct cells from exocrine glands.NEW & NOTEWORTHY This study addresses the uncertainty about the impact of CFTR on human salivary gland function. We found CFTR transcripts in a subset of duct cells known as ionocytes, as well as in acinar cells. Isolated human parotid acinar cells exhibited Cl- conductance consistent with CFTR activity. This marks the first documented evidence of functional CFTR expression in human salivary gland acinar cells.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Regulador de Condutância Transmembrana em Fibrose Cística / Células Acinares Tipo de estudo: Prognostic_studies Limite: Animals / Humans Idioma: En Revista: Am J Physiol Cell Physiol Assunto da revista: FISIOLOGIA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Chile País de publicação: Estados Unidos
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Regulador de Condutância Transmembrana em Fibrose Cística / Células Acinares Tipo de estudo: Prognostic_studies Limite: Animals / Humans Idioma: En Revista: Am J Physiol Cell Physiol Assunto da revista: FISIOLOGIA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Chile País de publicação: Estados Unidos