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Effects of polyphenolic maqui (Aristotelia chilensis) extract on the inhibition of NLRP3 inflammasome and activation of mast cells in a mouse model of Crohn's disease-like colitis.
Ortiz-Cerda, Tamara; Argüelles-Arias, Federico; Macías-García, Laura; Vázquez-Román, Victoria; Tapia, Gladys; Xie, Kangzhe; García-García, María Desirée; Merinero, Manuel; García-Montes, Josefa-María; Alcudia, Ana; Witting, Paul K; De-Miguel, Manuel.
Afiliação
  • Ortiz-Cerda T; Departamento de Citología e Histología Normal y Patológica, Facultad de medicina, Universidad de Sevilla, Seville, Spain.
  • Argüelles-Arias F; Redox Biology Group, The Charles Perkins Centre, School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, Sydney, NSW, Australia.
  • Macías-García L; Departamento de Medicina, Facultad de Medicina, Universidad de Sevilla, Seville, Spain.
  • Vázquez-Román V; Department of Gastroenterology, University Hospital Virgen Macarena, Seville, Spain.
  • Tapia G; Departamento de Citología e Histología Normal y Patológica, Facultad de medicina, Universidad de Sevilla, Seville, Spain.
  • Xie K; Departamento de Citología e Histología Normal y Patológica, Facultad de medicina, Universidad de Sevilla, Seville, Spain.
  • García-García MD; Molecular and Clinical Pharmacology, Institute of Biomedical Sciences, Faculty of Medicine, University of Chile, Santiago, Chile.
  • Merinero M; Redox Biology Group, The Charles Perkins Centre, School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, Sydney, NSW, Australia.
  • García-Montes JM; Department of Gastroenterology, University Hospital Virgen Macarena, Seville, Spain.
  • Alcudia A; Departamento de Citología e Histología Normal y Patológica, Facultad de medicina, Universidad de Sevilla, Seville, Spain.
  • Witting PK; Departamento de Química Orgánica y Farmacéutica, Universidad de Sevilla, Seville, Spain.
  • De-Miguel M; Departamento de Medicina, Facultad de Medicina, Universidad de Sevilla, Seville, Spain.
Front Immunol ; 14: 1229767, 2023.
Article em En | MEDLINE | ID: mdl-38283356
ABSTRACT

Introduction:

Crohn's disease (CD) involves activation of mast cells (MC) and NF-кB in parallel with the PPAR-α/NLRP3 inflammasome/IL-1ß pathway in the inflamed colon. Whether polyphenols from maqui (Aristotelia chilensis) represent a natural alternative treatment for CD is unclear. Therefore, we used an animal model of 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced CD-like colitis to investigate protective effects of maqui extract through monitoring NLRP3 inflammasome and MC activation in colon tissue.

Methods:

Maqui extract was administered via orogastric route to mice after (post-Treatment group) or prior (pre-Treatment group) to TNBS-induction. Colon pathology was characterized by histoarchitectural imaging, disease activity index (DAI), and assessing NF-кB, p-NF-кB, PPAR-α/NLRP3 expression and IL-1ß levels.

Results:

Compared to mice treated with TNBS alone administration of anthocyanin-rich maqui extract improved the DAI, colon histoarchitecture and reduced both colon wet-weight and transmural inflammation. Induction with TNBS significantly increased colonic NLPR3 inflammasome activation, while co-treatment with maqui extract (either post- or pre-Treatment) significantly downregulated NLRP3, ASC and caspase-1 levels, which manifested as reduced colonic IL-1ß levels. Supplemented maqui extract marginally diminished NF-кB activity in epithelial cells but reached statistical significance in immune cells (as judged by decreased NF-кB phosphorylation). PPAR-α signaling was largely unaffected by Maqui whereas MC infiltration into the colon mucosa and submucosa decreased and their level of degranulation was suppressed.

Conclusion:

These outcomes show the post- and pre- Treatment effect of a polyphenolic extract rich in anthocyanins from maqui the acute phase of TNBS- induced CD-like colitis is linked to suppression of the NLRP3 inflammasome and reduced MC responses. These data indicate that maqui extract represents a potential nutraceutical for the treatment of inflammatory bowel disease (IBD).
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Doença de Crohn / Colite / Antocianinas Tipo de estudo: Prognostic_studies Limite: Animals País/Região como assunto: America do sul / Chile Idioma: En Revista: Front Immunol Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Espanha País de publicação: Suíça
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Doença de Crohn / Colite / Antocianinas Tipo de estudo: Prognostic_studies Limite: Animals País/Região como assunto: America do sul / Chile Idioma: En Revista: Front Immunol Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Espanha País de publicação: Suíça