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Effect of quercetin, L-ergothioneine and H89 on sperm motility and kinematic pattern, plasma membrane functionality and in vitro heterologous fertilizing capacity of cryopreserved equine semen.
Lobo, Mariano Eliécer Acosta; Londoño, Guillermo Correa; Rojano, Benjamín Alberto; Betancur, Giovanni Restrepo.
Afiliação
  • Lobo MEA; Faculty of Veterinary Medicine and Zootechnics, Institución Universitaria Visión de las Américas Medellín, Colombia.
  • Londoño GC; Department of Agronomic Sciences. Faculty of Agricultural Sciences, Universidad Nacional de Colombia, Medellín, Colombia.
  • Rojano BA; Faculty of Sciences, Universidad Nacional de Colombia, Medellín, Colombia.
  • Betancur GR; Faculty of Agricultural Sciences, Universidad Nacional de Colombia, Cra 65 # 59A-110, Medellín, Colombia. Electronic address: grestre0@unal.edu.co.
J Equine Vet Sci ; 133: 105013, 2024 Feb.
Article em En | MEDLINE | ID: mdl-38281608
ABSTRACT
Semen cryopreservation causes extensive chemical and physical damage to sperm structure, which generates premature aging and reduces viability and fertility of spermatozoa. The addition of antioxidants to freezing extenders can reduce the oxidative damage caused by excessive generation of reactive oxygen species (ROS), and the premature aging could be reduced by adding an enzyme inhibitor that prevents an anticipated capacitation. The aim of this study was to evaluate the in vitro effect of quercetin (Q), L-ergothioneine (E) and H89 addition to cryopreserved equine spermatozoa. Six experimental groups were stablished control, Q, E, H89, H89Q and H89E. The analyzed parameters were sperm motility and kinematic using computer assisted sperm analysis (CASA), plasma membrane functionality with the hypoosmotic swelling test (HOST) and fertilizing capability with in vitro heterologous fertilization. Quercetin reduced curvilinear velocity (VCL) and increased beat-cross frequency (BCF), while its combination with H89 (H89Q) reduced total motility, progressive motility, VCL and hyperactive sperm (HA). Likewise, H89 and its combination with E (H89E) decreased VCL and amplitude of lateral head displacement (ALH). No significant differences were observed among treatments for membrane functionality and fertilizing capacity of sperm. In conclusion H89 in combination with Q and E reduced sperm motility or some kinematic parameters. However, they did not influence plasma membrane functionality and in vitro fertilizing capacity of frozen-thawed equine semen.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Sulfonamidas / Senilidade Prematura / Ergotioneína / Isoquinolinas Limite: Animals Idioma: En Revista: J Equine Vet Sci Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Colômbia País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Sulfonamidas / Senilidade Prematura / Ergotioneína / Isoquinolinas Limite: Animals Idioma: En Revista: J Equine Vet Sci Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Colômbia País de publicação: Estados Unidos