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Nationwide USA re-analysis of amplicon metabarcoding targeting ß-tubulin isoform-1 reveals absence of benzimidazole resistant SNPs in Ancylostoma braziliense, Ancylostoma tubaeforme and Uncinaria stenocephala.
Stocker, Thomas; Ward, Michael P; Slapeta, Jan.
Afiliação
  • Stocker T; Sydney School of Veterinary Science, Faculty of Science, The University of Sydney, New South Wales 2006, Australia.
  • Ward MP; Sydney School of Veterinary Science, Faculty of Science, The University of Sydney, New South Wales 2006, Australia.
  • Slapeta J; Sydney School of Veterinary Science, Faculty of Science, The University of Sydney, New South Wales 2006, Australia; The University of Sydney Institute for Infectious Diseases, New South Wales 2006, Australia. Electronic address: jan.slapeta@sydney.edu.au.
Vet Parasitol ; 327: 110118, 2024 Apr.
Article em En | MEDLINE | ID: mdl-38278035
ABSTRACT
Nationwide sampling by Venkatesan and colleagues (2023) described the resistance status of the canine hookworm, Ancylostoma caninum, to benzimidazoles across the USA via ß-tubulin isotype-1 amplicon metabarcoding. In this study, we aimed to use the existing public amplicon metabarcoding data and mine it for the presence of ß-tubulin isotype-1 sequences that belong to hookworm species other than A. caninum. Through bioinformatics analysis we assigned species to A. caninum, Ancylostoma braziliense, Ancylostoma tubaeforme and Uncinaria stenocephala. All non-A. caninum sequences contained only the benzimidazole susceptible residues of ß-tubulin isotype-1. Using two ß-tubulin isotype-1 metabarcoding sequence data (assay targeting 134 and 167 codons, and assay targeting 198 and 200 codons), 2.0% (6/307) and 2.9% (9/310) individual samples had hookworms other than A. caninum (A. braziliense n = 5, A. tubaeforme n = 4 and U. stenocephala n = 2), respectively. We identified one sample containing A. braziliense in each of the Northeastern region and Midwestern region, and in three samples from the Southern region. Presence of A. tubaeforme in dog faeces is considered as pseudoparasitism. There were no statistically significant regional differences for the distribution of each species, for either of the two assays independently or combined (χ2 tests, P > 0.05). Our work demonstrates the utility of the amplicon metabarcoding for the identification of species through antemortem assays, thus resolving the dilemma of assigning hookworm species based on either post-mortem or egg sizes for the identification of hookworms.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Doenças do Cão / Ancylostoma Limite: Animals País/Região como assunto: America do sul / Brasil Idioma: En Revista: Vet Parasitol Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Austrália País de publicação: Holanda
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Doenças do Cão / Ancylostoma Limite: Animals País/Região como assunto: America do sul / Brasil Idioma: En Revista: Vet Parasitol Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Austrália País de publicação: Holanda