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Endocannabinoid regulation in the cervix during pregnancy: insights into molecular mechanisms of premature labor.
Marvaldi, Carolina; Herrero, Felisa; Johnson, Clare; Aylen Schander, Julieta; Correa, Fernando; Cella, Maximiliano; Aisemberg, Julieta; Franchi, Ana María; Bradshaw, Heather; Wolfson, Manuel Luis.
Afiliação
  • Marvaldi C; Laboratorio de Fisiopatología de la Preñez y el Parto, Centro de Estudios Farmacológicos y Botánicos, Universidad de Buenos Aires-Consejo Nacional de Investigaciones en Ciencia y Técnica, Ciudad Autónoma de Buenos Aires, Argentina.
  • Herrero F; Laboratorio de Fisiopatología de la Preñez y el Parto, Centro de Estudios Farmacológicos y Botánicos, Universidad de Buenos Aires-Consejo Nacional de Investigaciones en Ciencia y Técnica, Ciudad Autónoma de Buenos Aires, Argentina.
  • Johnson C; Department of Psychological and Brain Sciences, Indiana University, Bloomington, Indiana, USA.
  • Aylen Schander J; Laboratorio de Fisiopatología de la Preñez y el Parto, Centro de Estudios Farmacológicos y Botánicos, Universidad de Buenos Aires-Consejo Nacional de Investigaciones en Ciencia y Técnica, Ciudad Autónoma de Buenos Aires, Argentina.
  • Correa F; Laboratorio de Fisiopatología de la Preñez y el Parto, Centro de Estudios Farmacológicos y Botánicos, Universidad de Buenos Aires-Consejo Nacional de Investigaciones en Ciencia y Técnica, Ciudad Autónoma de Buenos Aires, Argentina.
  • Cella M; Laboratorio de Fisiopatología de la Preñez y el Parto, Centro de Estudios Farmacológicos y Botánicos, Universidad de Buenos Aires-Consejo Nacional de Investigaciones en Ciencia y Técnica, Ciudad Autónoma de Buenos Aires, Argentina.
  • Aisemberg J; Laboratorio de Fisiopatología de la Preñez y el Parto, Centro de Estudios Farmacológicos y Botánicos, Universidad de Buenos Aires-Consejo Nacional de Investigaciones en Ciencia y Técnica, Ciudad Autónoma de Buenos Aires, Argentina.
  • Franchi AM; Laboratorio de Fisiopatología de la Preñez y el Parto, Centro de Estudios Farmacológicos y Botánicos, Universidad de Buenos Aires-Consejo Nacional de Investigaciones en Ciencia y Técnica, Ciudad Autónoma de Buenos Aires, Argentina.
  • Bradshaw H; Department of Psychological and Brain Sciences, Indiana University, Bloomington, Indiana, USA.
  • Wolfson ML; Laboratorio de Fisiopatología de la Preñez y el Parto, Centro de Estudios Farmacológicos y Botánicos, Universidad de Buenos Aires-Consejo Nacional de Investigaciones en Ciencia y Técnica, Ciudad Autónoma de Buenos Aires, Argentina.
Reproduction ; 167(4)2024 04 01.
Article em En | MEDLINE | ID: mdl-38271800
ABSTRACT
In brief The cervix plays a crucial role not only in the maintenance of pregnancy but also during delivery, when it undergoes extensive changes. This study highlights the involvement of the endocannabinoidome in cervical remodeling, emphasizing its relevance in the shift from a nonpregnant to pregnant state and its potential contribution to preterm delivery in inflammatory contexts. Abstract During pregnancy, the main role of the cervix is to isolate the fetus from outside pathogens and maintain the relatively closed system of uterine gestation. Conversely, toward the end of pregnancy, the cervix must be remodeled to increase flexibility and allow the delivery. This process is called cervical remodeling and dysregulation of the process plays a role in premature delivery. The endocannabinoidome plays an important role in several reproductive events; however, its function on cervical tissue throughout pregnancy is poorly understood. The goal of this study was to evaluate the presence and participation of the endocannabinoidome in lipopolysaccharide (LPS)-induced cervical changes. Therefore, we evaluated key components of the endocannabinoidome in cervical tissue from nonpregnant mice and pregnant mice with and without LPS treatment. Using mass spectrometric analysis, we found an increase in anandamide and 2-arachidonoylglycerol in the cervix of pregnant mice when compared to nonpregnant mice. We have also found a reduction in FAAH protein expression in these tissues. Furthermore, when treated with LPS, we observed a reduction in the cervical immunostaining with anti-CB1 and anti-CB2 antibodies. Likewise, using cervix explants from pregnant mice, we found that LPS significantly increased cervical metalloprotease activity and cyclooxygenase 2, which were subsequently modulated by cannabinoid receptor antagonists. Collectively, our findings suggest that an LPS-induced imbalance of cervix endocannabinoidome likely contributes to premature cervical remodeling, which is part of the key components that contribute to premature delivery.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Nascimento Prematuro / Trabalho de Parto Prematuro Limite: Animals / Female / Humans / Pregnancy Idioma: En Revista: Reproduction Assunto da revista: MEDICINA REPRODUTIVA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Argentina País de publicação: Reino Unido

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Nascimento Prematuro / Trabalho de Parto Prematuro Limite: Animals / Female / Humans / Pregnancy Idioma: En Revista: Reproduction Assunto da revista: MEDICINA REPRODUTIVA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Argentina País de publicação: Reino Unido