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Hypomethylation at H19DMR in penile squamous cell carcinoma is not related to HPV infection.
da Silva Santos, Renan; Pascoalino Pinheiro, Daniel; Gustavo Hirth, Carlos; Barbosa Bezerra, Maria Júlia; Joyce de Lima Silva-Fernandes, Isabelle; Andréa da Silva Oliveira, Francisca; Viana de Holanda Barros, Maisa; Silveira Ramos, Ester; A Moura, Arlindo; Filho, Odorico de Moraes Manoel; Pessoa, Claudia; Miranda Furtado, Cristiana Libardi.
Afiliação
  • da Silva Santos R; Department of Physiology and Pharmacology, Drug Research and Development Center, Federal University of Ceará, Fortaleza, Ceará, Brazil.
  • Pascoalino Pinheiro D; Oswaldo Cruz Foundation, FIOCRUZ-Ceará, Sector of Biotechnology, Eusebio, Ceará, Brazil.
  • Gustavo Hirth C; Laboratory of Pathology, Cancer Institute of Ceará, Fortaleza, Ceará, Brazil.
  • Barbosa Bezerra MJ; Laboratory of Molecular Biology and Genetics, Cancer Institute of Ceará, Fortaleza, Ceará, Brazil.
  • Joyce de Lima Silva-Fernandes I; Laboratory of Molecular Biology and Genetics, Cancer Institute of Ceará, Fortaleza, Ceará, Brazil.
  • Andréa da Silva Oliveira F; Genomics and Bioinformatics Center, Drug Research and Development Center, Federal University of Ceará, Fortaleza, Ceará, Brazil.
  • Viana de Holanda Barros M; Postgraduate Program in Translational Medicine, Drug Research and Development Center, Federal University of Ceará, Fortaleza, Ceará, Brazil.
  • Silveira Ramos E; Department of Genetics, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
  • A Moura A; Department of Physiology and Pharmacology, Drug Research and Development Center, Federal University of Ceará, Fortaleza, Ceará, Brazil.
  • Filho OMM; Department of Animal Science, Federal University of Ceará, Fortaleza, Ceará, Brazil.
  • Pessoa C; Department of Physiology and Pharmacology, Drug Research and Development Center, Federal University of Ceará, Fortaleza, Ceará, Brazil.
  • Miranda Furtado CL; Postgraduate Program in Translational Medicine, Drug Research and Development Center, Federal University of Ceará, Fortaleza, Ceará, Brazil.
Epigenetics ; 19(1): 2305081, 2024 Dec.
Article em En | MEDLINE | ID: mdl-38245880
ABSTRACT
Penile squamous cell carcinoma (SCC) is a rare and aggressive tumour mainly related to lifestyle behaviour and human papillomavirus (HPV) infection. Environmentally induced loss of imprinting (LOI) at the H19 differentially methylated region (H19DMR) is associated with many cancers in the early events of tumorigenesis and may be involved in the pathogenesis of penile SCC. We sought to evaluate the DNA methylation pattern at H19DMR and its association with HPV infection in men with penile SCC by bisulfite sequencing (bis-seq). We observed an average methylation of 32.2% ± 11.6% at the H19DMR of penile SCC and did not observe an association between the p16INK4a+ (p = 0.59) and high-risk HPV+ (p = 0.338) markers with methylation level. The average methylation did not change according to HPV positive for p16INK4a+ or hrHPV+ (35.4% ± 10%) and negative for both markers (32.4% ± 10.1%) groups. As the region analysed has a binding site for the CTCF protein, the hypomethylation at the surrounding CpG sites might alter its insulator function. In addition, there was a positive correlation between intense polymorphonuclear cell infiltration and hypomethylation at H19DMR (p = 0.035). Here, we report that hypomethylation at H19DMR in penile SCC might contribute to tumour progression and aggressiveness regardless of HPV infection.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Carcinoma de Células Escamosas / Infecções por Papillomavirus / RNA Longo não Codificante Limite: Humans / Male Idioma: En Revista: Epigenetics Assunto da revista: GENETICA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
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PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Carcinoma de Células Escamosas / Infecções por Papillomavirus / RNA Longo não Codificante Limite: Humans / Male Idioma: En Revista: Epigenetics Assunto da revista: GENETICA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos