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Compact CRISPR genetic screens enabled by improved guide RNA library cloning.
Heo, Seok-Jin; Enriquez, Lauren D; Federman, Scot; Chang, Amy Y; Mace, Rachel; Shevade, Kaivalya; Nguyen, Phuong; Litterman, Adam J; Shafer, Shawn; Przybyla, Laralynne; Chow, Eric D.
Afiliação
  • Heo SJ; Laboratory for Genomics Research, San Francisco, CA, 94158, USA.
  • Enriquez LD; Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA, 94158, USA.
  • Federman S; Laboratory for Genomics Research, San Francisco, CA, 94158, USA.
  • Chang AY; Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA, 94158, USA.
  • Mace R; Laboratory for Genomics Research, San Francisco, CA, 94158, USA.
  • Shevade K; Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA, 94158, USA.
  • Nguyen P; Laboratory for Genomics Research, San Francisco, CA, 94158, USA.
  • Litterman AJ; Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA, 94158, USA.
  • Shafer S; Laboratory for Genomics Research, San Francisco, CA, 94158, USA.
  • Przybyla L; Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA, 94158, USA.
  • Chow ED; Laboratory for Genomics Research, San Francisco, CA, 94158, USA.
Genome Biol ; 25(1): 25, 2024 01 19.
Article em En | MEDLINE | ID: mdl-38243310
ABSTRACT
CRISPR genome editing approaches theoretically enable researchers to define the function of each human gene in specific cell types, but challenges remain to efficiently perform genetic perturbations in relevant models. In this work, we develop a library cloning protocol that increases sgRNA uniformity and greatly reduces bias in existing genome-wide libraries. We demonstrate that our libraries can achieve equivalent or better statistical power compared to previously reported screens using an order of magnitude fewer cells. This improved cloning protocol enables genome-scale CRISPR screens in technically challenging cell models and screen formats.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Sistemas CRISPR-Cas / RNA Guia de Sistemas CRISPR-Cas Limite: Humans Idioma: En Revista: Genome Biol Assunto da revista: BIOLOGIA MOLECULAR / GENETICA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Estados Unidos País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Sistemas CRISPR-Cas / RNA Guia de Sistemas CRISPR-Cas Limite: Humans Idioma: En Revista: Genome Biol Assunto da revista: BIOLOGIA MOLECULAR / GENETICA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Estados Unidos País de publicação: Reino Unido