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Inorganic Polyphosphate Affects Biofilm Assembly, Capsule Formation, and Virulence of Hypervirulent ST23 Klebsiella pneumoniae.
Rojas, Diego; Marcoleta, Andrés E; Gálvez-Silva, Matías; Varas, Macarena A; Díaz, Mauricio; Hernández, Mauricio; Vargas, Cristian; Nourdin-Galindo, Guillermo; Koch, Elard; Saldivia, Pablo; Vielma, Jorge; Gan, Yunn-Hwen; Chen, Yahua; Guiliani, Nicolás; Chávez, Francisco P.
Afiliação
  • Rojas D; Laboratorio de Microbiología de Sistemas, Departamento de Biología, Facultad de Ciencias, Universidad de Chile, Santiago CP 7800003, Chile.
  • Marcoleta AE; Grupo de Microbiología Integrativa, Laboratorio de Biología Estructural y Molecular, Departamento de Biología, Facultad de Ciencias, Universidad de Chile, Santiago CP 7800003, Chile.
  • Gálvez-Silva M; Laboratorio de Microbiología de Sistemas, Departamento de Biología, Facultad de Ciencias, Universidad de Chile, Santiago CP 7800003, Chile.
  • Varas MA; Grupo de Microbiología Integrativa, Laboratorio de Biología Estructural y Molecular, Departamento de Biología, Facultad de Ciencias, Universidad de Chile, Santiago CP 7800003, Chile.
  • Díaz M; Laboratorio de Microbiología de Sistemas, Departamento de Biología, Facultad de Ciencias, Universidad de Chile, Santiago CP 7800003, Chile.
  • Hernández M; Grupo de Microbiología Integrativa, Laboratorio de Biología Estructural y Molecular, Departamento de Biología, Facultad de Ciencias, Universidad de Chile, Santiago CP 7800003, Chile.
  • Vargas C; Laboratorio de Comunicación Microbiana, Departamento de Biología, Facultad de Ciencias, Universidad de Chile, Santiago CP 7800003, Chile.
  • Nourdin-Galindo G; División Biotecnología, Instituto Melisa, San Pedro de la Paz CP 9660000, Chile.
  • Koch E; División Biotecnología, Instituto Melisa, San Pedro de la Paz CP 9660000, Chile.
  • Saldivia P; División Biotecnología, Instituto Melisa, San Pedro de la Paz CP 9660000, Chile.
  • Vielma J; División Biotecnología, Instituto Melisa, San Pedro de la Paz CP 9660000, Chile.
  • Gan YH; División Biotecnología, Instituto Melisa, San Pedro de la Paz CP 9660000, Chile.
  • Chen Y; Facultad de Ciencias Biológicas, Universidad de Concepción, Concepción CP 4070389, Chile.
  • Guiliani N; Laboratorio de Microbiología de Sistemas, Departamento de Biología, Facultad de Ciencias, Universidad de Chile, Santiago CP 7800003, Chile.
  • Chávez FP; Grupo de Microbiología Integrativa, Laboratorio de Biología Estructural y Molecular, Departamento de Biología, Facultad de Ciencias, Universidad de Chile, Santiago CP 7800003, Chile.
ACS Infect Dis ; 10(2): 606-623, 2024 02 09.
Article em En | MEDLINE | ID: mdl-38205780
ABSTRACT
The emergence of hypervirulent Klebsiella pneumoniae (hvKP) strains poses a significant threat to public health due to high mortality rates and propensity to cause severe community-acquired infections in healthy individuals. The ability to form biofilms and produce a protective capsule contributes to its enhanced virulence and is a significant challenge to effective antibiotic treatment. Polyphosphate kinase 1 (PPK1) is an enzyme responsible for inorganic polyphosphate synthesis and plays a vital role in regulating various physiological processes in bacteria. In this study, we investigated the impact of polyP metabolism on the biofilm and capsule formation and virulence traits in hvKP using Dictyostelium discoideum amoeba as a model host. We found that the PPK1 null mutant was impaired in biofilm and capsule formation and showed attenuated virulence in D. discoideum compared to the wild-type strain. We performed a proteomic analysis to gain further insights into the underlying molecular mechanism. The results revealed that the PPK1 mutant had a differential expression of proteins involved in capsule synthesis (Wzi-Ugd), biofilm formation (MrkC-D-H), synthesis of the colibactin genotoxin precursor (ClbB), as well as proteins associated with the synthesis and modification of lipid A (ArnB-LpxC-PagP). These proteomic findings corroborate the phenotypic observations and indicate that the PPK1 mutation is associated with impaired biofilm and capsule formation and attenuated virulence in hvKP. Overall, our study highlights the importance of polyP synthesis in regulating extracellular biomolecules and virulence in K. pneumoniae and provides insights into potential therapeutic targets for treating K. pneumoniae infections.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Dictyostelium / Klebsiella pneumoniae Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Revista: ACS Infect Dis Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Chile País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Dictyostelium / Klebsiella pneumoniae Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Revista: ACS Infect Dis Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Chile País de publicação: Estados Unidos