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Kinematic and Morphometric Assessment of Fresh Semen, before, during and after Mating Period in Brahman Bulls.
Araya-Zúñiga, Ignacio; Sevilla, Francisco; Molina-Montero, Rafael; Roldan, Eduardo R S; Barrientos-Morales, Manuel; Silvestre, Miguel A; Valverde, Anthony.
Afiliação
  • Araya-Zúñiga I; Laboratory of Animal Reproduction, School of Agronomy, Costa Rica Institute of Technology, San Carlos Campus, Alajuela 223-21002, Costa Rica.
  • Sevilla F; Laboratory of Animal Reproduction, School of Agronomy, Costa Rica Institute of Technology, San Carlos Campus, Alajuela 223-21002, Costa Rica.
  • Molina-Montero R; Natural Sciences for Development (DOCINADE), Costa Rica Institute of Technology, San Carlos Campus, Alajuela 223-21002, Costa Rica.
  • Roldan ERS; Laboratory of Animal Reproduction, School of Agronomy, Costa Rica Institute of Technology, San Carlos Campus, Alajuela 223-21002, Costa Rica.
  • Barrientos-Morales M; Department of Biodiversity and Evolutionary Biology, National Museum of Natural Sciences, Spanish National Research Council (CSIC), 28006 Madrid, Spain.
  • Silvestre MA; Laboratory of Reproductive Biology, School of Veterinary Medicine and Animal Science, University of Veracruz, Veracruz CP 91710, Mexico.
  • Valverde A; Department of Cellular Biology, Functional Biology and Physical Anthropology, Campus Burjassot, University of Valencia, C/Dr Moliner 50, 46100 Burjassot, Spain.
Animals (Basel) ; 14(1)2023 Dec 29.
Article em En | MEDLINE | ID: mdl-38200863
ABSTRACT
The objective of the present study was to determine the effects that the reproductive season has on the motility, kinematics, morphology, and sperm morphometry of Brahman bulls evaluated with a commercial CASA system. The experiment was carried out at the Costa Rica Institute of Technology from March to August 2021. A total of eight Brahman bulls were used. A total of 28 ejaculates were collected in the pre-mating period (PMP), during it (DMP), and after it (AMP) using an electroejaculator. The sperm concentration was measured with the Accuread photometer. The motility was measured using a Spermtrack® counting chamber. The analyses were performed with the CASA-Mot ISAS®v1 system. The morphology was analyzed using a microscope with a negative phase contrast objective. Morphometry was evaluated with the CASA-Morph. The sperm concentration did not present differences between the PMP and AMP; however, it was significantly higher than DMP (p > 0.05). Regarding the progressiveness variables, linearity on forward progression (LIN), straightness (STR), and wobble (WOB) were higher (p < 0.05) DMP. A kinematic principal component analysis grouped all the variables into three factors and an effect on the reproductive period was found (p < 0.05) in the parameters of the head and middle part of the sperm, such as width and perimeter, which were greater in the PMP. The length of the sperm head in the PMP and DMP did not show differences; however, both were larger (p < 0.05) than AMP. The insertion distance of the middle piece of the sperm was significantly greater than DMP. Finally, the PMP contained cells with a larger insertion angle (p < 0.05) than AMP. These findings are important to understand the implications of reproductive status on sperm quality and to consider them in andrological evaluations.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Animals (Basel) Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Costa Rica País de publicação: Suíça
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Animals (Basel) Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Costa Rica País de publicação: Suíça