Cytotoxicity evaluation, antibacterial effect, and degree of conversion of QAM-containing adhesives.

Garcia, Isadora Martini; Assad-Loss, Tatiana Féres; Schneider, Luis Felipe Jochinms; Collares, Fabrício Mezzomo; Cavalcante, Larissa Maria Assad; Tostes, Mônica Almeida

Garcia, Isadora Martini; Assad-Loss, Tatiana Féres; Schneider, Luis Felipe Jochinms; Collares, Fabrício Mezzomo; Cavalcante, Larissa Maria Assad; Tostes, Mônica Almeida.

Afiliação

Garcia IM; University of Maryland School of Dentistry, Department of General Dentistry, Baltimore, MD, USA.
Assad-Loss TF; Universidade Federal Fluminense - UFF, School of Dentistry, Graduate Program in Dentistry, Federal Fluminense University, Niterói, RJ, Brazil.
Schneider LFJ; Universidade Federal Fluminense - UFF, School of Dentistry, Graduate Program in Dentistry, Federal Fluminense University, Niterói, RJ, Brazil.
Collares FM; Universidade Federal do Rio Grande do Sul - UFRGS, School of Dentistry, Laboratory of Dental Materials, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.
Cavalcante LMA; Universidade Federal Fluminense - UFF, School of Dentistry, Graduate Program in Dentistry, Federal Fluminense University, Niterói, RJ, Brazil.
Tostes MA; Universidade Federal Fluminense - UFF, School of Dentistry, Graduate Program in Dentistry, Federal Fluminense University, Niterói, RJ, Brazil.

Braz Oral Res ; 38: e001, 2024.

Article em En | MEDLINE | ID: mdl-38198301

ABSTRACT

ABSTRACT

The aim of this study was to evaluate the influence of adding quaternary ammonium methacrylates (QAMs) to experimental adhesives by assessing the degree of conversion (DC), cytotoxicity against keratinocytes and fibroblasts, and antibacterial activity against biofilm formation. Two QAMs were added to an experimental adhesive dimethylaminododecyl methacrylate bromododecane (DMADDM) or dimethylaminododecyl methacrylate bromohexadecane (DMAHDM) at three concentrations each 1, 2.5, and 5 wt.%. Experimental adhesive without QAMs (control group) and commercially available Transbond XT Primer (3M Unitek, Monrovia, California, USA) were used for comparisons. The adhesives were tested for DC, cytotoxicity against keratinocytes and fibroblasts, and antibacterial activity against biofilm formation. DC, cytotoxicity against fibroblasts, and antibacterial activity were analyzed using one-way ANOVA and Tukey's multiple comparisons. Cytotoxicity against keratinocytes was evaluated using the Kruskal Wallis and Dunn's post-hoc (α = 5%) tests. Transbond showed lower DC as compared to 5% DMAHDM, 1% DMADDM, and 5% DMADDM (p < 0.05). However, all groups presented proper DC when compared to commercial adhesives in the literature. In the evaluation of cytotoxicity against keratinocytes, Transbond induced higher viability than 2.5 wt.% groups (p < 0.05). Against fibroblasts, Transbond induced higher viability as compared to 5 wt.% groups (p < 0.05). DMAHDM at 5 wt.% reduced biofilm formation when compared to all the other groups (p < 0.05). Despite their cytotoxic effect against keratinocytes, gingival fibroblasts showed higher viability. DMAHDM at 5 wt.% decreased Streptococcus mutans viability. The incorporation of DMAHDM at 5 wt.% may be a strategy for reducing the development of white spot lesions.

Assuntos

Antibacterianos; Bis-Fenol A-Glicidil Metacrilato; Hidrocarbonetos Bromados; Metacrilatos; Metilaminas; Compostos de Amônio Quaternário; Metacrilatos/farmacologia; Antibacterianos/farmacologia

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Bis-Fenol A-Glicidil Metacrilato / Compostos de Amônio Quaternário / Hidrocarbonetos Bromados / Metacrilatos / Metilaminas / Antibacterianos Idioma: En Revista: Braz Oral Res Assunto da revista: ODONTOLOGIA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Estados Unidos País de publicação: Brasil

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