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Foodborne Viruses and Somatic Coliphages Occurrence in Fresh Produce at Retail from Northern Mexico.
Ossio, Axel; Flores-Rodríguez, Fernanda; Heredia, Norma; García, Santos; Merino-Mascorro, Jose Angel.
Afiliação
  • Ossio A; Laboratorio de Bioquímica y Genética de Microorganismos, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, 66455, San Nicolas de los Garza, N.L., Mexico.
  • Flores-Rodríguez F; Laboratorio de Bioquímica y Genética de Microorganismos, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, 66455, San Nicolas de los Garza, N.L., Mexico.
  • Heredia N; Laboratorio de Bioquímica y Genética de Microorganismos, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, 66455, San Nicolas de los Garza, N.L., Mexico.
  • García S; Laboratorio de Bioquímica y Genética de Microorganismos, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, 66455, San Nicolas de los Garza, N.L., Mexico.
  • Merino-Mascorro JA; Laboratorio de Bioquímica y Genética de Microorganismos, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, 66455, San Nicolas de los Garza, N.L., Mexico. jose.merinomsc@uanl.edu.mx.
Food Environ Virol ; 16(1): 109-119, 2024 Mar.
Article em En | MEDLINE | ID: mdl-38198031
ABSTRACT
Foodborne disease outbreaks linked to consumption of vegetables have been often attributed to human enteric viruses, such as Norovirus (NoV), Hepatitis A virus (HAV), and Rotavirus (RoV). Information about the occurrence of these viruses is scarce in many fresh-producing countries. Viral contamination detection of indicators, such as somatic coliphages, could indirectly reflect the presence of viral pathogens, being a valuable tool for better viral risk assessment in food industry. This study aimed to establish the occurrence and correlation of foodborne viruses and somatic coliphages in leafy greens in northern Mexico. A total of 320 vegetable samples were collected, resulting in 80 composite rinses, 40 of lettuce and 40 of parsley. Somatic coliphages were determined using the EPA 1602 method, while foodborne viruses (HAV, RoV, NoV GI, and GII) were determined by qPCR. The occurrence of RoV was 22.5% (9/40, mean 2.11 log gc/g) in lettuce and 20% (8/40, mean 1.91 log gc/g) in parsley. NoV and HAV were not detected in any samples. Somatic coliphages were present in all lettuce and parsley samples, with mean levels of 1.85 log PFU/100 ml and 2.28 log PFU/100 ml, respectively. Spearman analysis established the correlation of somatic coliphages and genomic copies of RoV, resulting in an r2 value of - 0.026 in lettuce and 0.349 in parsley. Although NoV or HAV were undetected in the samples, the presence of RoV is a matter of concern as leafy greens are usually eaten raw, which poses a potential risk of infection.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Vírus / Enterovirus / Rotavirus / Vírus da Hepatite A / Norovirus Tipo de estudo: Risk_factors_studies Limite: Humans País/Região como assunto: Mexico Idioma: En Revista: Food Environ Virol Ano de publicação: 2024 Tipo de documento: Article País de afiliação: México País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Vírus / Enterovirus / Rotavirus / Vírus da Hepatite A / Norovirus Tipo de estudo: Risk_factors_studies Limite: Humans País/Região como assunto: Mexico Idioma: En Revista: Food Environ Virol Ano de publicação: 2024 Tipo de documento: Article País de afiliação: México País de publicação: Estados Unidos