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Osteogenic organoid for bone regeneration: Healing of bone defect in congenital pseudoarthrosis of the tibia.
Cardier, Jose E; Diaz-Solano, Dylana; Wittig, Olga; Sierra, Giuseppe; Pulido, Jose; Moreno, Rita; Fuentes, Soraima; Leal, Fredy.
Afiliação
  • Cardier JE; Unidad de Terapia Celular-Laboratorio de Patología Celular y Molecular, Centro de Medicina Regenerativa, Instituto Venezolano de Investigaciones Científicas (IVIC), Caracas, Venezuela.
  • Diaz-Solano D; Unidad de Terapia Celular-Laboratorio de Patología Celular y Molecular, Centro de Medicina Regenerativa, Instituto Venezolano de Investigaciones Científicas (IVIC), Caracas, Venezuela.
  • Wittig O; Unidad de Terapia Celular-Laboratorio de Patología Celular y Molecular, Centro de Medicina Regenerativa, Instituto Venezolano de Investigaciones Científicas (IVIC), Caracas, Venezuela.
  • Sierra G; Servicio de Traumatología, Hospital Pediátrico Niño Jesús, Yaracuy, Venezuela.
  • Pulido J; Servicio de Traumatología, Hospital Dr. Plácido Rodríguez Rivero, Yaracuy, Venezuela.
  • Moreno R; Servicio de Traumatología, Hospital Dr. Plácido Rodríguez Rivero, Yaracuy, Venezuela.
  • Fuentes S; Servicio de Traumatología, Hospital Pediátrico Niño Jesús, Yaracuy, Venezuela.
  • Leal F; Servicio de Traumatología, Hospital Pediátrico Niño Jesús, Yaracuy, Venezuela.
Int J Artif Organs ; 47(2): 107-114, 2024 Feb.
Article em En | MEDLINE | ID: mdl-38182554
ABSTRACT

BACKGROUND:

Congenital pseudoarthrosis of the tibia (CPT) is an uncommon disease associated with failure to achieve bone union and recurrent fractures. There is evidence showing that CPT is associated with decreased osteogenesis. Based on the capacity of mesenchymal stromal cells (MSCs) to induce osteogenesis, we develop an osteogenic organoid (OstO) constituted by these cells, and other components of the bone niche, for inducing bone formation in a child diagnosed with CPT.

AIM:

To evaluate the capacity of an OstO to induce bone formation in a patient with CPT.

METHODS:

The OstO was fabricated with allogeneic bone marrow MSCs from a healthy donor, collagen microbeads (CM) and PRP clot. The CM and PRP function as extracellular matrix and scaffolds for MSC. The OstO was placed at the site of non-union. Internal and external fixation was placed in the tibia. Radiological evaluation was performed after MSCs transplantation.

RESULTS:

After 4 months of MSCs transplantation, radiographic imaging showed evidence of osteogenesis at the site of CPT lesion. The tibia showed bone consolidation and complete healing of the non-union CPT lesion after 6 months. Functional improvement was observed after 1 year of MSC transplantation.

CONCLUSIONS:

The OstO is a bone-like niche which promote osteogenesis in patients with failure in bone formation, such as CPT. To our knowledge, these results provide the first evidence showing CPT healing induced by an OstO constituted by allogeneic MSCs. Future studies incorporating a larger number of patients may confirm these results.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Osteogênese / Pseudoartrose / Tíbia Limite: Child / Humans Idioma: En Revista: Int J Artif Organs Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Venezuela País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Osteogênese / Pseudoartrose / Tíbia Limite: Child / Humans Idioma: En Revista: Int J Artif Organs Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Venezuela País de publicação: Estados Unidos