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(1 â†’ 3),(1 â†’ 6) and (1 â†’ 3)-ß-D-glucan physico-chemical features drive their fermentation profile by the human gut microbiota.
Zavadinack, Matheus; Cantu-Jungles, Thaisa M; Abreu, Hellen; Ozturk, Oguz K; Cordeiro, Lucimara M C; de Freitas, Rilton A; Hamaker, Bruce R; Iacomini, Marcello.
Afiliação
  • Zavadinack M; Department of Biochemistry and Molecular Biology, Federal University of Paraná, Curitiba, PR, CEP 81531-980, Brazil.
  • Cantu-Jungles TM; Whistler Center for Carbohydrate Research, Department of Food Science, Purdue University, West Lafayette, IN 47907, USA.
  • Abreu H; Department of Biochemistry and Molecular Biology, Federal University of Paraná, Curitiba, PR, CEP 81531-980, Brazil.
  • Ozturk OK; Whistler Center for Carbohydrate Research, Department of Food Science, Purdue University, West Lafayette, IN 47907, USA.
  • Cordeiro LMC; Department of Biochemistry and Molecular Biology, Federal University of Paraná, Curitiba, PR, CEP 81531-980, Brazil.
  • de Freitas RA; Department of Pharmacy Federal University of Paraná, Curitiba, PR CEP 80210-170, Brazil.
  • Hamaker BR; Whistler Center for Carbohydrate Research, Department of Food Science, Purdue University, West Lafayette, IN 47907, USA.
  • Iacomini M; Department of Biochemistry and Molecular Biology, Federal University of Paraná, Curitiba, PR, CEP 81531-980, Brazil. Electronic address: iacomini@ufpr.br.
Carbohydr Polym ; 327: 121678, 2024 Mar 01.
Article em En | MEDLINE | ID: mdl-38171663
ABSTRACT
Mushroom polysaccharides consist of a unique set of polymers that arrive intact in the human large intestine becoming available for fermentation by resident gut bacteria with potential benefits to the host. Here we have obtained four glucans from two mushrooms (Pholiota nameko and Pleurotus pulmonarius) under different extraction conditions and their fermentation profile by human gut bacteria in vitro was evaluated. These glucans were isolated and characterized as (1 â†’ 3),(1 â†’ 6)-ß-D-glucans varying in branching pattern and water-solubility. An aliquot of each (1 â†’ 3),(1 â†’ 6)-ß-D-glucan was subjected to controlled smith degradation process in order to obtain a linear (1 â†’ 3)-ß-D-glucan from each fraction. The four ß-D-glucans demonstrated different water solubilities and molar mass ranging from 2.2 × 105 g.mol-1 to 1.9 × 106 g.mol-1. In vitro fermentation of the glucans by human gut microbiota showed they induced different short chain fatty acid production (52.0-97.0 mM/50 mg carbohydrates), but an overall consistent high propionate amount (28.5-30.3 % of total short chain fatty acids produced). All glucans promoted Bacteroides uniformis, whereas Anaerostipes sp. and Bacteroides ovatus promotion was strongly driven by the ß-D-glucans solubility and/or branching pattern, highlighting the importance of ß-D-glucan discrete structures to their fermentation by the human gut microbiota.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Beta-Glucanas / Microbioma Gastrointestinal Limite: Humans Idioma: En Revista: Carbohydr Polym Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Beta-Glucanas / Microbioma Gastrointestinal Limite: Humans Idioma: En Revista: Carbohydr Polym Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido