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Antiproliferative and Apoptosis Effects of Hybrid Varieties of Vitis vinifera L. Sweet Sapphire and Sweet Surprise on Human Prostate Cancer Cells Using In Vitro and In Silico Approaches.
Cruz, Marta Angela De Almeida Sousa; Pascoal, Gabriela De Freitas Laiber; Jacintho, Maria Eduarda De Souza; Wagner, Maria Luisa Barambo; Coimbra, Pedro Paulo Saldanha; Araujo-Lima, Carlos Fernando; Junior, Antonio Palumbo; Teodoro, Anderson Junger.
Afiliação
  • Cruz MAAS; Food and Nutrition Program, Functional Foods Laboratory, Federal University of the State of Rio de Janeiro, Rio de Janeiro, Brazil.
  • Pascoal GFL; Department of Food and Experimental Nutrition, Pharmaceutical Sciences School, University of Sao Paulo, São Paulo, Brazil.
  • Jacintho MES; Functional Foods Laboratory, Federal University of the State of Rio de Janeiro, Rio de Janeiro, Brazil.
  • Wagner MLB; Laboratory of Cellular Interactions, Biomedical Sciences Institute, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
  • Coimbra PPS; Food and Nutrition Program, Functional Foods Laboratory, Federal University of the State of Rio de Janeiro, Rio de Janeiro, Brazil.
  • Araujo-Lima CF; Food and Nutrition Program, Functional Foods Laboratory, Federal University of the State of Rio de Janeiro, Rio de Janeiro, Brazil.
  • Junior AP; Department of Genetics and Molecular Biology, Federal University of the State of Rio de Janeiro, Rio de Janeiro, Brazil.
  • Teodoro AJ; Laboratory of Cellular Interactions, Biomedical Sciences Institute, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
Asian Pac J Cancer Prev ; 24(11): 3673-3684, 2023 Nov 01.
Article em En | MEDLINE | ID: mdl-38019224
OBJECTIVE: Grape hybrids are characterized by different chemical compositions; often with high hybrids are characterized by different chemical compositions, often with a high phenolic content and a specific profile of anthocyanins. The aim of study was to characterize the constituents of hybrid Vitis vinifera L. varieties Sweet Sapphire (SA) and Sweet Surprise (SU) extracts and their influence on apoptosis induction and antiproliferative effects on human prostate cancer cells. METHODS: We used the MTT assay to evaluate the cytotoxic effect of extracts of SA and SU, on the prostate adenocarcinoma cell lines PC-3 and DU-145. To analyze the inhibiting impact by flow cytometry, used 24 and 48 hours. Anthocyanins were quantified by liquid chromatography and analysed by their absorption rate, hepatotoxicity, blood concentration, blood-brain barrier passage ability and maximum recommended dose by in silico approaches. RESULTS: Our results showed that malvidin derivatives present the highest content in both cultivars. We identified 14.46mg/100g malvidin-3-O-glycoside in SA and 2.76 mg/100 g in SU. A reduction in cell viability of DU-145 (45 and 65%) and PC-3 (63 and 67%) cells after 48h treatment with SA and SU, respectively, was found via MTT assay. Flow cytometry showed that the treatment with extracts from SA and SU had an inhibitory impact on cell development due to G2/M arrest and caused a rise in apoptotic cells compared to control group. None of the anthocyanin presented hepatotoxicity as well as blood-brain barrier passage ability. Peonidin 3-O-glucoside had the lower maximum recommended dose as well as the highest intestinal absorption rate. However, delphinidin 3-O-glucoside had the highest blood concentration values. CONCLUSION: The findings of this study highlight the potential of hybrid Vitis vinifera L. varieties as an important source of natural antioxidants and their protective effect against prostate cancer cells as well as elucidate in part their anthocyanin's metabolism.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias da Próstata / Vitis / Doença Hepática Induzida por Substâncias e Drogas Limite: Humans / Male Idioma: En Revista: Asian Pac J Cancer Prev Assunto da revista: NEOPLASIAS Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Brasil País de publicação: Tailândia

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias da Próstata / Vitis / Doença Hepática Induzida por Substâncias e Drogas Limite: Humans / Male Idioma: En Revista: Asian Pac J Cancer Prev Assunto da revista: NEOPLASIAS Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Brasil País de publicação: Tailândia