Your browser doesn't support javascript.
loading
Leveraging the use of ionic liquid capillary columns and GC×GC-MS for fatty acid profiling in human colostrum samples.
Dias, Fernanda Furlan Goncalves; Bogusz, Stanislau; Silva, Racire Sampaio; Fronza, Marcio; Hantao, Leandro Wang.
Afiliação
  • Dias FFG; Institute of Chemistry, University of Campinas (Unicamp), Campinas, São Paulo, Brazil.
  • Bogusz S; Department of Food Science and Nutrition (FScN), University of Minnesota, Twin Cities (UMN), St. Paul, Minnesota, MN, USA.
  • Silva RS; University of São Paulo (USP), São Carlos Institute of Chemistry (IQSC), São Carlos, São Paulo, Brazil. stanislau@iqsc.usp.br.
  • Fronza M; Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Vila Velha, Vila Velha, Brazil.
  • Hantao LW; Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Vila Velha, Vila Velha, Brazil.
Anal Bioanal Chem ; 416(1): 191-201, 2024 Jan.
Article em En | MEDLINE | ID: mdl-37924376
Lipids in human colostrum provide the majority of energy intake and essential fatty acids for developing infants. The fatty acid composition of human colostrum is highly variable and influenced by multiple factors. Human colostrum is a complex sample bringing challenges to fatty acid profiling. This work aimed to optimize the use of ionic liquid (IL) columns and flow-modulated comprehensive two-dimensional gas chromatography coupled to mass spectrometry (FM-GC×GC-MS) for fatty acid profiling in human colostrum. Derivatization strategies were optimized and the elution behavior of fatty acid methyl esters (FAME) on various 1D column phases (Solgel-WAX, SLB-IL60i, SLB-IL76i, and SLB-IL111i). Derivatization with sodium methoxide yielded a satisfactory recovery rate (90%) at milder conditions and reduced time. The use of IL60 as the 1D column provided superior separation, good peak shape, and better utilization of elution space. As a proof of concept, the developed method was applied to access the effects of the mode of neonatal delivery (vaginal vs. C-section) on the fatty acid profile of human colostrum samples. The integrated multidimensional gas chromatography strategy improved FAME detection and separation and can be a useful tool for accessing the effects of different factors on the fatty acid profiling of complex samples.
Assuntos
Palavras-chave
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Líquidos Iônicos / Ácidos Graxos Limite: Female / Humans / Newborn / Pregnancy Idioma: En Revista: Anal Bioanal Chem Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Alemanha
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Líquidos Iônicos / Ácidos Graxos Limite: Female / Humans / Newborn / Pregnancy Idioma: En Revista: Anal Bioanal Chem Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Alemanha