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Acid tolerance of Lactobacillus spp. on root carious lesions: A complex and multifaceted response.
Santos, Heitor Sales de Barros; Damé-Teixeira, Nailê; Nagano, Martina Hitomi; Do, Thuy; Parolo, Clarissa Cavalcanti Fatturi; Maltz, Marisa; Arthur, Rodrigo Alex.
Afiliação
  • Santos HSB; Preventive and Community Dentistry Department, Dental School, Federal University of Rio Grande do Sul (UFRGS), Ramiro Barcelos, 2492, Porto Alegre 90035-003, Brazil.
  • Damé-Teixeira N; Department of Dentistry, School of Health Sciences, University of Brasilia, Campus Universitario Darcy Ribeiro, 70910-900 Brasilia, Brazil; Division of Oral Biology, School of Dentistry, University of Leeds, Wellcome Trust Brenner Building, St. James' University Hospital, LS9 7TF Leeds, United Kingd
  • Nagano MH; Preventive and Community Dentistry Department, Dental School, Federal University of Rio Grande do Sul (UFRGS), Ramiro Barcelos, 2492, Porto Alegre 90035-003, Brazil.
  • Do T; Division of Oral Biology, School of Dentistry, University of Leeds, Wellcome Trust Brenner Building, St. James' University Hospital, LS9 7TF Leeds, United Kingdom.
  • Parolo CCF; Preventive and Community Dentistry Department, Dental School, Federal University of Rio Grande do Sul (UFRGS), Ramiro Barcelos, 2492, Porto Alegre 90035-003, Brazil.
  • Maltz M; Preventive and Community Dentistry Department, Dental School, Federal University of Rio Grande do Sul (UFRGS), Ramiro Barcelos, 2492, Porto Alegre 90035-003, Brazil.
  • Arthur RA; Preventive and Community Dentistry Department, Dental School, Federal University of Rio Grande do Sul (UFRGS), Ramiro Barcelos, 2492, Porto Alegre 90035-003, Brazil. Electronic address: rodrigoarthur.ufrgs@gmail.com.
Arch Oral Biol ; 156: 105820, 2023 Dec.
Article em En | MEDLINE | ID: mdl-37866118
Lactobacillus spp. are acidogenic and aciduric bacteria and are among the main cariogenic microorganisms associated with the carious process. OBJECTIVE: This study aimed to identify genes involved in the acid-tolerance of Lactobacillus spp. and potential functions attributed to these genes within the metatranscriptome of sound root surfaces and carious root surfaces. DESIGN: Genomic libraries were built from mRNA isolated from the biofilm samples (10 from sound root and 9 from carious root using Illumina HiSeq 2500). Reads generated by RNA-seq were mapped against 162 oral microbial genomes and genes potentially related to acid tolerance were manually extracted from the Lactobacillus spp. genomes using L. paracasei ATCC 344 as reference genome. The R package DESeq2 was used to calculate the level of differential gene expression between those two clinical conditions. RESULTS: Fifteen Lactobacillus spp. genomes were identified and a total of 653 acid tolerance genes were overexpressed in carious root surfaces. Multiple functions, as translation, ribosomal structure and biogenesis, transport of nucleotides and amino acids, are involved in Lactobacillus spp. acid tolerance. Species-specific functions also seem to be related to the fitness of Lactobacillus spp. in acidified environments such as that of the cariogenic biofilm associated with carious root lesions. CONCLUSIONS: The response of Lactobacillus spp. to an acidic environment is complex and multifaceted. This finding suggests several possible avenues for further research into the adaptive mechanisms of these bacteria.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Cárie Dentária / Lactobacillus Limite: Humans Idioma: En Revista: Arch Oral Biol Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Cárie Dentária / Lactobacillus Limite: Humans Idioma: En Revista: Arch Oral Biol Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido