Tracking the sources of Leptospira and nutrient flows in two urban watersheds of Puerto Rico.

Chapman, Taylor; Bachoon, D S; Martinez, G A; Burt, C D; DeMontigny, Wesley C

Chapman, Taylor; Bachoon, D S; Martinez, G A; Burt, C D; DeMontigny, Wesley C.

Afiliação

Chapman T; Department of Biological and Environmental Sciences, Georgia College and State University, Campus Box 81, Milledgeville, GA, 31061-0490, USA.
Bachoon DS; Department of Biological and Environmental Sciences, Georgia College and State University, Campus Box 81, Milledgeville, GA, 31061-0490, USA. dave.bachoon@gcsu.edu.
Martinez GA; University of Puerto Rico, Mayagüez Campus, San Juan, Puerto Rico.
Burt CD; Department of Biological and Environmental Sciences, Georgia College and State University, Campus Box 81, Milledgeville, GA, 31061-0490, USA.
DeMontigny WC; Department of Biological and Environmental Sciences, Georgia College and State University, Campus Box 81, Milledgeville, GA, 31061-0490, USA.

Environ Monit Assess ; 195(11): 1318, 2023 Oct 14.

Article em En | MEDLINE | ID: mdl-37833564

RESUMO

This study investigated the relationship between nutrient levels, source of fecal contamination, and pathogenic Leptospira in Puerto Rico's northern coast and San Juan Bay Estuary (SJBE) aquatic ecosystems. Microbial source tracking (MST) was also used to investigate the connections between sources of feces contamination and the presence of Leptospira. Eighty-seven water samples were collected during the June (n=44) and August (n=43) in 2020. To quantify phosphorus and nitrogen concentrations, standard USEPA protocols were utilized, specifically Methods 365.4 for total and dissolved phosphorus, 351.2 for total Kjeldahl nitrogen and ammonium, and 353.2 for nitrate. Lipl32 gene-specific quantitative polymerase chain reaction (qPCR) was used to detect the presence of Leptospira. Human (HF183), canine (BacCan-UCD), and equine (HoF597) MST assays were utilized to trace the origins of fecal contamination. Forty one percent of the locations exceeded Puerto Rico's authorized total phosphorus limit of 160 g L-1, while 34% exceeded the total nitrogen limit of 1700 g L-1. Nearly half of the streams examined are affected by eutrophication. The MST analysis identified human and canine feces as the most prevalent contaminants, affecting approximately 50% of the sites. In addition, Leptospira was detected in 32% of the June samples. There was a significant correlation (r = 0.79) between the incidence of pathogenic Leptospira and the human bacterial marker (HF183). This study illuminates the central role of anthropogenic inputs in nutrient enrichment and pathogen proliferation in Puerto Rico's aquatic ecosystems.

Assuntos

Monitoramento Ambiental; Leptospira; Humanos; Cavalos; Animais; Cães; Monitoramento Ambiental/métodos; Poluição da Água/análise; Porto Rico; Ecossistema; Microbiologia da Água; Fezes/microbiologia; Nitrogênio/análise; Fósforo/análise

Palavras-chave

Fecal pollution; Leptospira; Microbial source tracking; Puerto Rico

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Monitoramento Ambiental / Leptospira Limite: Animals / Humans País/Região como assunto: Caribe / Puerto rico Idioma: En Revista: Environ Monit Assess Assunto da revista: SAUDE AMBIENTAL Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Estados Unidos País de publicação: Holanda

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