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Physical exercise elicits UPRmt in the skeletal muscle: The role of c-Jun N-terminal kinase.
Gaspar, Rodrigo Stellzer; Katashima, Carlos Kiyoshi; Crisol, Barbara Moreira; Carneiro, Fernanda Silva; Sampaio, Igor; Silveira, Leonardo Dos Reis; Silva, Adelino Sanchez Ramos da; Cintra, Dennys Esper; Pauli, José Rodrigo; Ropelle, Eduardo Rochete.
Afiliação
  • Gaspar RS; Laboratory of Molecular Biology of Exercise (LaBMEx), School of Applied Sciences (FCA), University of Campinas (Unicamp), Limeira, Brazil; Laboratory of Cell Signaling, Obesity and Comorbidities Research Center (OCRC), University of Campinas (Unicamp), Campinas, São Paulo, Brazil.
  • Katashima CK; Laboratory of Molecular Biology of Exercise (LaBMEx), School of Applied Sciences (FCA), University of Campinas (Unicamp), Limeira, Brazil.
  • Crisol BM; Laboratory of Molecular Biology of Exercise (LaBMEx), School of Applied Sciences (FCA), University of Campinas (Unicamp), Limeira, Brazil.
  • Carneiro FS; Laboratory of Molecular Biology of Exercise (LaBMEx), School of Applied Sciences (FCA), University of Campinas (Unicamp), Limeira, Brazil.
  • Sampaio I; Department of Structural and Functional Biology, Biology Institute, University of Campinas (Unicamp), Campinas, Brazil.
  • Silveira LDR; Department of Structural and Functional Biology, Biology Institute, University of Campinas (Unicamp), Campinas, Brazil.
  • Silva ASRD; School of Physical Education and Sport of Ribeirão Preto, University of São Paulo (USP), Ribeirão Preto, São Paulo, Brazil.
  • Cintra DE; Laboratory of Nutritional Genomics (Labgen), School of Applied Sciences (FCA), University of Campinas (Unicamp), Limeira, Brazil.
  • Pauli JR; Laboratory of Molecular Biology of Exercise (LaBMEx), School of Applied Sciences (FCA), University of Campinas (Unicamp), Limeira, Brazil.
  • Ropelle ER; Laboratory of Molecular Biology of Exercise (LaBMEx), School of Applied Sciences (FCA), University of Campinas (Unicamp), Limeira, Brazil; Faculty of Medical Sciences, Department of Internal Medicine. University of Campinas (Unicamp), Campinas, São Paulo, Brazil. Electronic address: eduardoropelle@g
Mol Metab ; 78: 101816, 2023 Dec.
Article em En | MEDLINE | ID: mdl-37821006
OBJECTIVE: The mitochondrial unfolded protein response (UPRmt) is an adaptive cellular response to stress to ensure mitochondrial proteostasis and function. Here we explore the capacity of physical exercise to induce UPRmt in the skeletal muscle. METHODS: Therefore, we combined mouse models of exercise (swimming and treadmill running), pharmacological intervention, and bioinformatics analyses. RESULTS: Firstly, RNA sequencing and Western blotting analysis revealed that an acute aerobic session stimulated several mitostress-related genes and protein content in muscle, including the UPRmt markers. Conversely, using a large panel of isogenic strains of BXD mice, we identified that BXD73a and 73b strains displayed low levels of several UPRmt-related genes in the skeletal muscle, and this genotypic feature was accompanied by body weight gain, lower locomotor activity, and aerobic capacity. Finally, we identified that c-Jun N-terminal kinase (JNK) activation was critical in exercise-induced UPRmt in the skeletal muscle since pharmacological JNK pathway inhibition blunted exercise-induced UPRmt markers in mice muscle. CONCLUSION: Our findings provide new insights into how exercise triggers mitostress signals toward the oxidative capacity in the skeletal muscle.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Condicionamento Físico Animal / Proteínas Quinases JNK Ativadas por Mitógeno Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Mol Metab Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Brasil País de publicação: Alemanha
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Condicionamento Físico Animal / Proteínas Quinases JNK Ativadas por Mitógeno Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Mol Metab Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Brasil País de publicação: Alemanha