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Reusing wastewater from Coffea arabica processing to produce single-cell protein using Candida sorboxylosa: Optimizing of culture conditions.
Pillaca-Pullo, Omar Santiago; Lopes, André Moreni; Estela-Escalante, Waldir D.
Afiliação
  • Pillaca-Pullo OS; Facultad de Química e Ingeniería Química, Laboratorio de Bioprocesos y Tecnología de Fermentación, Universidad Nacional Mayor de San Marcos, Lima, Peru.
  • Lopes AM; Department of Biotechnology, Lorena School of Engineering, University of São Paulo (EEL/USP), Lorena, Brazil.
  • Estela-Escalante WD; Facultad de Química e Ingeniería Química, Laboratorio de Bioprocesos y Tecnología de Fermentación, Universidad Nacional Mayor de San Marcos, Lima, Peru.
Biotechnol Prog ; 40(1): e3393, 2024.
Article em En | MEDLINE | ID: mdl-37792408
Coffee is a crop of significant socioeconomic importance, and the reuse of agri-food by-products and biowaste has great potential across several industries. Coffee wastewater (CWW) is a valuable resource containing essential nutrients that can be utilized by Candida sorboxylosa for single-cell protein (SCP) production. This utilization contributes to mitigating the negative impacts of agro-industrial waste. The optimization of culture conditions using the design of experiments (DoE) technique is crucial in understanding the environmental factors influencing metabolite production. In our study, the DoE technique was employed to analyze culture conditions, including room temperature, pH 8.4, agitation at 200 rpm, a headspace of 60% (v/v), and an inoculum of 0.75 DO600nm over 28-h period. This approach resulted in a remarkable SCP yield of 64.4% and dry cell weight (DCW) of 2.26 g/L. It is noteworthy that there is no literature reporting SCP production under alkaline pH conditions in yeast. Interestingly, our work demonstrated that an alkaline pH of 8.4 significantly influenced SCP production by C. sorboxylosa. The DoE technique proved to be an efficient statistical tool for optimizing culture conditions, offering several advantages, such as: (i) conducting cultures at room temperature to minimize unnecessary energy consumption; (ii) reducing the incubation time from 46 to 28 h, thereby enhancing overall productivity; (iii) achieving 1.7-fold increase in SCP yield compared to previous basal production levels.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Candida / Coffea Idioma: En Revista: Biotechnol Prog Assunto da revista: BIOTECNOLOGIA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Peru País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Candida / Coffea Idioma: En Revista: Biotechnol Prog Assunto da revista: BIOTECNOLOGIA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Peru País de publicação: Estados Unidos